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[[Image:1cbf.gif|left|200px]]<br /><applet load="1cbf" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1cbf, resolution 2.4&Aring;" />
'''THE X-RAY STRUCTURE OF A COBALAMIN BIOSYNTHETIC ENZYME, COBALT PRECORRIN-4 METHYLTRANSFERASE, CBIF'''<br />


==Overview==
==THE X-RAY STRUCTURE OF A COBALAMIN BIOSYNTHETIC ENZYME, COBALT PRECORRIN-4 METHYLTRANSFERASE, CBIF==
Biosynthesis of the corrin ring of vitamin B12 requires the action of six S-adenosyl-L-methionine (AdoMet) dependent transmethylases, closely related in sequence. The first X-ray structure of one of these, cobalt-precorrin-4 transmethylase, CbiF, from Bacillus megaterium has been determined to a resolution of 2.4 A. CbiF contains two alphabeta domains forming a trough in which S-adenosyl-L-homocysteine (AdoHcy) binds. The location of AdoHcy and a number of conserved residues, helps define the precorrin binding site. A second crystal form determined at 3.1 A resolution highlights the flexibility of two loops around this site. CbiF employs a unique mode of AdoHcy binding and represents a new class of transmethylase.
<StructureSection load='1cbf' size='340' side='right'caption='[[1cbf]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1cbf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Priestia_megaterium Priestia megaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CBF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CBF FirstGlance]. <br>
1CBF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_megaterium Bacillus megaterium] with <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=SAH:'>SAH</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Precorrin-4_C(11)-methyltransferase Precorrin-4 C(11)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.133 2.1.1.133] Known structural/functional Sites: <scene name='pdbsite=PAL:Phosphate+Ion+Involved+In+Crystal+Contact'>PAL</scene> and <scene name='pdbsite=SAH:S-Adenosyl-Homocysteine+Binding+Site.+Crystals+Formed+In+...'>SAH</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CBF OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cbf OCA], [https://pdbe.org/1cbf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cbf RCSB], [https://www.ebi.ac.uk/pdbsum/1cbf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cbf ProSAT]</span></td></tr>
The X-ray structure of a cobalamin biosynthetic enzyme, cobalt-precorrin-4 methyltransferase., Schubert HL, Wilson KS, Raux E, Woodcock SC, Warren MJ, Nat Struct Biol. 1998 Jul;5(7):585-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9665173 9665173]
</table>
[[Category: Bacillus megaterium]]
== Function ==
[[Category: Precorrin-4 C(11)-methyltransferase]]
[https://www.uniprot.org/uniprot/CBIF_PRIMG CBIF_PRIMG] Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5A.<ref>PMID:23922391</ref>
[[Category: Single protein]]
== Evolutionary Conservation ==
[[Category: Raux, E.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Schubert, H L.]]
Check<jmol>
[[Category: Warren, M J.]]
  <jmolCheckbox>
[[Category: Wilson, K S.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cb/1cbf_consurf.spt"</scriptWhenChecked>
[[Category: Woodcock, S C.]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: PO4]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: SAH]]
  </jmolCheckbox>
[[Category: cobalamin biosynthesis]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cbf ConSurf].
[[Category: methylase]]
<div style="clear:both"></div>
[[Category: precorrin-4 methyltransferase]]
== References ==
 
<references/>
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:04:20 2008''
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Priestia megaterium]]
[[Category: Raux E]]
[[Category: Schubert HL]]
[[Category: Warren MJ]]
[[Category: Wilson KS]]
[[Category: Woodcock SC]]
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