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[[Image:1cfr.gif|left|200px]]<br /><applet load="1cfr" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1cfr, resolution 2.15&Aring;" />
'''CRYSTAL STRUCTURE OF CITROBACTER FREUNDII RESTRICTION ENDONUCLEASE CFR10I AT 2.15 ANGSTROMS RESOLUTION.'''<br />


==Overview==
==CRYSTAL STRUCTURE OF CITROBACTER FREUNDII RESTRICTION ENDONUCLEASE CFR10I AT 2.15 ANGSTROMS RESOLUTION.==
The X-ray crystal structure of Citrobacter freundii restriction endonuclease Cfr10I has been determined at a resolution of 2.15 A by multiple isomorphous replacement methods and refined to an R-factor of 19.64%. The structure of Cfr10I represents the first structure of a restriction endonuclease recognizing a degenerated nucleotide sequence. Structural comparison of Cfr10I with previously solved structures of other restriction enzymes suggests that recognition of specific sequence occurs through contacts in the major and the minor grooves of DNA. The arrangement of the putative active site residues shows some striking differences from previously described restriction endonucleases and supports a two-metal-ion mechanism of catalysis.
<StructureSection load='1cfr' size='340' side='right'caption='[[1cfr]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1cfr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Citrobacter_freundii Citrobacter freundii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CFR FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cfr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cfr OCA], [https://pdbe.org/1cfr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cfr RCSB], [https://www.ebi.ac.uk/pdbsum/1cfr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cfr ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/T2CX_CITFR T2CX_CITFR] Recognizes the double-stranded sequence RCCGGY and cleaves after R-1.


==About this Structure==
==See Also==
1CFR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Citrobacter_freundii Citrobacter freundii]. Active as [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFR OCA].
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Crystal structure of Citrobacter freundii restriction endonuclease Cfr10I at 2.15 A resolution., Bozic D, Grazulis S, Siksnys V, Huber R, J Mol Biol. 1996 Jan 12;255(1):176-86. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8568865 8568865]
[[Category: Citrobacter freundii]]
[[Category: Citrobacter freundii]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Type II site-specific deoxyribonuclease]]
[[Category: Bozic D]]
[[Category: Bozic, D.]]
[[Category: Grazulis S]]
[[Category: Grazulis, S.]]
[[Category: Huber R]]
[[Category: Huber, R.]]
[[Category: Siksnys V]]
[[Category: Siksnys, V.]]
[[Category: restriction endonuclease]]
[[Category: restriction enzyme]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:05:37 2008''

Latest revision as of 06:42, 7 February 2024

CRYSTAL STRUCTURE OF CITROBACTER FREUNDII RESTRICTION ENDONUCLEASE CFR10I AT 2.15 ANGSTROMS RESOLUTION.

1cfr, resolution 2.15Å

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