|
|
| (11 intermediate revisions by the same user not shown) |
| Line 1: |
Line 1: |
| [[Image:1d32.jpg|left|200px]]
| |
|
| |
|
| {{Structure
| | ==DRUG-INDUCED DNA REPAIR: X-RAY STRUCTURE OF A DNA-DITERCALINIUM COMPLEX== |
| |PDB= 1d32 |SIZE=350|CAPTION= <scene name='initialview01'>1d32</scene>, resolution 1.700Å
| | <StructureSection load='1d32' size='340' side='right'caption='[[1d32]], [[Resolution|resolution]] 1.70Å' scene=''> |
| |SITE=
| | == Structural highlights == |
| |LIGAND= <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DIT:DITERCALINIUM'>DIT</scene>
| | <table><tr><td colspan='2'>[[1d32]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D32 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D32 FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> |
| |GENE=
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DIT:DITERCALINIUM'>DIT</scene></td></tr> |
| |DOMAIN=
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d32 OCA], [https://pdbe.org/1d32 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d32 RCSB], [https://www.ebi.ac.uk/pdbsum/1d32 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d32 ProSAT]</span></td></tr> |
| |RELATEDENTRY=
| | </table> |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1d32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d32 OCA], [http://www.ebi.ac.uk/pdbsum/1d32 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1d32 RCSB]</span>
| | __TOC__ |
| }}
| | </StructureSection> |
| | | [[Category: Large Structures]] |
| '''DRUG-INDUCED DNA REPAIR: X-RAY STRUCTURE OF A DNA-DITERCALINIUM COMPLEX'''
| | [[Category: Chen S-L]] |
| | | [[Category: Egli M]] |
| | | [[Category: Gao Q]] |
| ==Overview==
| | [[Category: Quigley GJ]] |
| Ditercalinium is a synthetic anticancer drug that binds to DNA by bis-intercalation and activates DNA repair processes. In prokaryotes, noncovalent DNA-ditercalinium complexes are incorrectly recognized by the uvrABC repair system as covalent lesions on DNA. In eukaryotes, mitochondrial DNA is degraded by excess and futile DNA repair. Using x-ray crystallography, we have determined, to 1.7 A resolution, the three-dimensional structure of a complex of ditercalinium bound to the double-stranded DNA fragment [d(CGCG)]2. The DNA in the complex with ditercalinium is kinked (by 15 degrees) and severely unwound (by 36 degrees) with exceptionally wide major and minor grooves. Recognition of the DNA-ditercalinium complex by uvrABC in prokaryotes, and by mitochondrial DNA repair systems in eukaryotes, might be related to drug-induced distortion of the DNA helix.
| | [[Category: Rabinovich D]] |
| | | [[Category: Rich A]] |
| ==About this Structure==
| | [[Category: Williams LD]] |
| 1D32 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D32 OCA].
| |
| | |
| ==Reference==
| |
| Drug-induced DNA repair: X-ray structure of a DNA-ditercalinium complex., Gao Q, Williams LD, Egli M, Rabinovich D, Chen SL, Quigley GJ, Rich A, Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2422-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/2006181 2006181]
| |
| [[Category: Protein complex]] | |
| [[Category: Chen, S L.]] | |
| [[Category: Egli, M.]] | |
| [[Category: Gao, Q.]] | |
| [[Category: Quigley, G J.]] | |
| [[Category: Rabinovich, D.]] | |
| [[Category: Rich, A.]] | |
| [[Category: Williams, L D.]] | |
| [[Category: complexed with drug]]
| |
| [[Category: double helix]]
| |
| [[Category: right handed dna]]
| |
| | |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:33:40 2008''
| |
