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| [[Image:1d90.gif|left|200px]]<br /><applet load="1d90" size="350" color="white" frame="true" align="right" spinBox="true"
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| caption="1d90, resolution 1.700Å" />
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| '''REFINED CRYSTAL STRUCTURE OF AN OCTANUCLEOTIDE DUPLEX WITH I.T MISMATCHED BASE PAIRS'''<br />
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| ==Overview== | | ==REFINED CRYSTAL STRUCTURE OF AN OCTANUCLEOTIDE DUPLEX WITH I.T MISMATCHED BASE PAIRS== |
| The structure of the synthetic deoxyoctamer d(GGIGCTCC) has been determined by single crystal X-ray diffraction techniques to a resolution of 1.7A. The sequence crystallises in space group P6(1), with unit cell dimensions a = b = 45.07, c = 45.49A. The refinement converged with a crystallographic residual R = 0.14 and the location of 81 solvent molecules. The octamer forms an A-DNA duplex with 6 Watson-Crick (G.C) base pairs and 2 inosine-thymine (I.T) pairs. Refinement of the structure shows it to be essentially isomorphous with that reported for d(GGGGCTCC) with the mispairs adopting a "wobble" conformation. Conformational parameters and base stacking interactions are compared to those for the native duplex d(GGGGCCCC) and other similar sequences. A rationale for the apparent increased crystal packing efficiency and lattice stability of the I.T octamer is given.
| | <StructureSection load='1d90' size='340' side='right'caption='[[1d90]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | | == Structural highlights == |
| ==About this Structure== | | <table><tr><td colspan='2'>[[1d90]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D90 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D90 FirstGlance]. <br> |
| 1D90 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D90 OCA].
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> |
| | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d90 OCA], [https://pdbe.org/1d90 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d90 RCSB], [https://www.ebi.ac.uk/pdbsum/1d90 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d90 ProSAT]</span></td></tr> |
| ==Reference== | | </table> |
| Refined crystal structure of an octanucleotide duplex with I.T. mismatched base pairs., Cruse WB, Aymani J, Kennard O, Brown T, Jack AG, Leonard GA, Nucleic Acids Res. 1989 Jan 11;17(1):55-72. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2911488 2911488]
| | __TOC__ |
| [[Category: Protein complex]] | | </StructureSection> |
| [[Category: Aymani, J.]]
| | [[Category: Large Structures]] |
| [[Category: Brown, T.]] | | [[Category: Aymani J]] |
| [[Category: Cruse, W B.T.]] | | [[Category: Brown T]] |
| [[Category: Jack, A G.C.]] | | [[Category: Cruse WBT]] |
| [[Category: Kennard, O.]] | | [[Category: Jack AGC]] |
| [[Category: Leonard, G A.]] | | [[Category: Kennard O]] |
| [[Category: a-dna]] | | [[Category: Leonard GA]] |
| [[Category: double helix]] | |
| [[Category: mismatched]] | |
| [[Category: modified]] | |
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:14:13 2008''
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