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[[Image:1kfo.gif|left|200px]]


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==CRYSTAL STRUCTURE OF AN RNA HELIX RECOGNIZED BY A ZINC-FINGER PROTEIN: AN 18 BASE PAIR DUPLEX AT 1.6 RESOLUTION==
The line below this paragraph, containing "STRUCTURE_1kfo", creates the "Structure Box" on the page.
<StructureSection load='1kfo' size='340' side='right'caption='[[1kfo]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1kfo]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KFO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KFO FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5BU:5-BROMO-URIDINE-5-MONOPHOSPHATE'>5BU</scene></td></tr>
{{STRUCTURE_1kfo|  PDB=1kfo  |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kfo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kfo OCA], [https://pdbe.org/1kfo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kfo RCSB], [https://www.ebi.ac.uk/pdbsum/1kfo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kfo ProSAT]</span></td></tr>
 
</table>
'''CRYSTAL STRUCTURE OF AN RNA HELIX RECOGNIZED BY A ZINC-FINGER PROTEIN: AN 18 BASE PAIR DUPLEX AT 1.6 RESOLUTION'''
__TOC__
 
</StructureSection>
 
[[Category: Large Structures]]
==Overview==
[[Category: Hattman S]]
The crystal structure of the 19-mer RNA, 5'-GAAUGCCUGCGAGCAUCCC-3' has been determined from X-ray diffraction data to 1.6 A resolution by the multiwavelength anomalous diffraction method from crystals containing a brominated uridine. In the crystal, this RNA forms an 18-mer self-complementary double helix with the 19th nucleotide flipped out of the helix. This helix contains most of the target stem recognized by the bacteriophage Mu Com protein (control of mom), which activates translation of an unusual DNA modification enzyme, Mom. The 19-mer duplex, which contains one A.C mismatch and one A.C/G.U tandem wobble pair, was shown to bind to the Com protein by native gel electrophoresis shift assay. Comparison of the geometries and base stacking properties between Watson-Crick base pairs and the mismatches in the crystal structure suggest that both hydrogen bonding and base stacking are important for stabilizing these mismatched base pairs, and that the unusual geometry adopted by the A.C mismatch may reveal a unique structural motif required for the function of Com.
[[Category: Hildenbrand J]]
 
[[Category: Korostelev A]]
==About this Structure==
[[Category: Li H]]
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KFO OCA].  
[[Category: Lima S]]
 
==Reference==
Crystal structure of an RNA helix recognized by a zinc-finger protein: an 18-bp duplex at 1.6 A resolution., Lima S, Hildenbrand J, Korostelev A, Hattman S, Li H, RNA. 2002 Jul;8(7):924-32. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12166647 12166647]
[[Category: Hattman, S.]]
[[Category: Hildenbrand, J.]]
[[Category: Korostelev, A.]]
[[Category: Li, H.]]
[[Category: Lima, S.]]
[[Category: Rna]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 22:41:25 2008''

Latest revision as of 07:46, 7 February 2024

CRYSTAL STRUCTURE OF AN RNA HELIX RECOGNIZED BY A ZINC-FINGER PROTEIN: AN 18 BASE PAIR DUPLEX AT 1.6 RESOLUTION

1kfo, resolution 1.60Å

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