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[[Image:1mli.gif|left|200px]]


{{Structure
==CRYSTAL STRUCTURE OF MUCONOLACTONE ISOMERASE AT 3.3 ANGSTROMS RESOLUTION==
|PDB= 1mli |SIZE=350|CAPTION= <scene name='initialview01'>1mli</scene>, resolution 3.3&Aring;
<StructureSection load='1mli' size='340' side='right'caption='[[1mli]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[1mli]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MLI FirstGlance]. <br>
|ACTIVITY= [http://en.wikipedia.org/wiki/Muconolactone_Delta-isomerase Muconolactone Delta-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.4 5.3.3.4]
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
|GENE=  
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mli OCA], [https://pdbe.org/1mli PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mli RCSB], [https://www.ebi.ac.uk/pdbsum/1mli PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mli ProSAT]</span></td></tr>
}}
</table>
 
== Function ==
'''CRYSTAL STRUCTURE OF MUCONOLACTONE ISOMERASE AT 3.3 ANGSTROMS RESOLUTION'''
[https://www.uniprot.org/uniprot/CATC_PSEPU CATC_PSEPU]
 
== Evolutionary Conservation ==
 
[[Image:Consurf_key_small.gif|200px|right]]
==Overview==
Check<jmol>
The crystal structure of muconolactone isomerase from Pseudomonas putida, a unique molecule with ten 96 amino acid subunits and 5-fold, and 2-fold symmetries, has been solved at 3.3 A resolution. The non-crystallographic symmetries were used to refine the initial single isomorphous replacement phases and produce an interpretable 10-fold averaged map. The backbone trace is complete and confirmed by the amino acid sequence fit. Each subunit is composed of a body with two alpha-helices and an antiparallel twisted beta-sheet of four strands, and an extended arm. The helices and the sheet fold to form a two-layered structure with an enclosed hydrophobic core and a partially formed putative active site pocket. The C-terminal arm of another subunit related by a local dyad symmetry extends over the core to complete this pocket. The decameric protein is almost spherical, with the helices forming the external coat. There is a large hydrophilic cavity in the center with open ends along the 5-fold axis. Molecular interactions between subunits are extensive. Each subunit contacts four neighbors and loses nearly 40% of its solvent contact area on oligomerization.
  <jmolCheckbox>
 
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ml/1mli_consurf.spt"</scriptWhenChecked>
==About this Structure==
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
1MLI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLI OCA].  
    <text>to colour the structure by Evolutionary Conservation</text>
 
  </jmolCheckbox>
==Reference==
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mli ConSurf].
Crystal structure of muconolactone isomerase at 3.3 A resolution., Katti SK, Katz BA, Wyckoff HW, J Mol Biol. 1989 Feb 5;205(3):557-71. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/2926818 2926818]
<div style="clear:both"></div>
[[Category: Muconolactone Delta-isomerase]]
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas putida]]
[[Category: Pseudomonas putida]]
[[Category: Single protein]]
[[Category: Katti SK]]
[[Category: Katti, S K.]]
[[Category: Katz BA]]
[[Category: Katz, B A.]]
[[Category: Wyckoff HW]]
[[Category: Wyckoff, H W.]]
[[Category: intramolecular oxidoreductase]]
 
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Retrieved from "https://proteopedia.org/1mli"