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New page: left|200px<br /><applet load="1on2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1on2, resolution 1.61Å" /> '''Bacillus subtilis Ma...
 
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[[Image:1on2.gif|left|200px]]<br /><applet load="1on2" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1on2, resolution 1.61&Aring;" />
'''Bacillus subtilis Manganese Transport Regulator (MntR), D8M Mutant, Bound to Manganese'''<br />


==Overview==
==Bacillus subtilis Manganese Transport Regulator (MntR), D8M Mutant, Bound to Manganese==
The Bacillus subtilis manganese transport regulator, MntR, binds Mn2+ as, an effector and is a repressor of transporters that import manganese. A, member of the diphtheria toxin repressor (DtxR) family of, metalloregulatory proteins, MntR exhibits selectivity for Mn2+ over Fe2+., Replacement of a metal-binding residue, Asp8, with methionine (D8M), relaxes this specificity. We report here the X-ray crystal structures of, wild-type MntR and the D8M mutant bound to manganese with 1.75 A and 1.61, A resolution, respectively. The 142-residue MntR homodimer has substantial, structural similarity to the 226-residue DtxR but lacks the C-terminal, SH3-like domain of DtxR. The metal-binding pockets of MntR and DtxR are, substantially different. The cation-to-cation distance between the two, manganese ions bound by MntR is 3.3 A, whereas that between the metal ions, bound by DtxR is 9 A. D8M binds only a single Mn2+ per monomer, owing to, alteration of the metal-binding site. The sole retained metal site adopts, pseudo-hexacoordinate geometry rather than the pseudo-heptacoordinate, geometry of the MntR metal sites.
<StructureSection load='1on2' size='340' side='right'caption='[[1on2]], [[Resolution|resolution]] 1.61&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1on2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ON2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ON2 FirstGlance]. <br>
1ON2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with MN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ON2 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.61&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1on2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1on2 OCA], [https://pdbe.org/1on2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1on2 RCSB], [https://www.ebi.ac.uk/pdbsum/1on2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1on2 ProSAT]</span></td></tr>
Structure of the manganese-bound manganese transport regulator of Bacillus subtilis., Glasfeld A, Guedon E, Helmann JD, Brennan RG, Nat Struct Biol. 2003 Aug;10(8):652-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12847518 12847518]
</table>
== Function ==
[https://www.uniprot.org/uniprot/MNTR_BACSU MNTR_BACSU] Central regulator of manganese homeostasis. In the presence of manganese, it mediates repression of the manganese transporter MntH; under low manganese conditions, it activates the transcription of the mntABCD operon.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/on/1on2_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1on2 ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Brennan, R.G.]]
[[Category: Brennan RG]]
[[Category: Glasfeld, A.]]
[[Category: Glasfeld A]]
[[Category: Guedon, E.]]
[[Category: Guedon E]]
[[Category: Helmann, J.D.]]
[[Category: Helmann JD]]
[[Category: MN]]
[[Category: dna-binding protein]]
[[Category: helix-turn-helix]]
[[Category: metalloregulatory protein]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:01:27 2007''
Retrieved from "https://proteopedia.org/1on2"