1p74: Difference between revisions

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New page: left|200px<br /><applet load="1p74" size="450" color="white" frame="true" align="right" spinBox="true" caption="1p74, resolution 2.40Å" /> '''CRYSTAL STRUCTURE OF...
 
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[[Image:1p74.jpg|left|200px]]<br /><applet load="1p74" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1p74, resolution 2.40&Aring;" />
'''CRYSTAL STRUCTURE OF SHIKIMATE DEHYDROGENASE (AROE) FROM HAEMOPHILUS INFLUENZAE'''<br />


==Overview==
==CRYSTAL STRUCTURE OF SHIKIMATE DEHYDROGENASE (AROE) FROM HAEMOPHILUS INFLUENZAE==
Shikimate dehydrogenase catalyzes the NADPH-dependent reversible reduction, of 3-dehydroshikimate to shikimate. We report the first X-ray structure of, shikimate dehydrogenase from Haemophilus influenzae to 2.4-A resolution, and its complex with NADPH to 1.95-A resolution. The molecule contains two, domains, a catalytic domain with a novel open twisted alpha/beta motif and, an NADPH binding domain with a typical Rossmann fold. The enzyme contains, a unique glycine-rich P-loop with a conserved sequence motif, GAGGXX, that, results in NADPH adopting a nonstandard binding mode with the nicotinamide, and ribose moieties disordered in the binary complex. A deep pocket with a, narrow entrance between the two domains, containing strictly conserved, residues primarily contributed by the catalytic domain, is identified as a, potential 3-dehydroshikimate binding pocket. The flexibility of the, nicotinamide mononucleotide portion of NADPH may be necessary for the, substrate 3-dehydroshikimate to enter the pocket and for the release of, the product shikimate.
<StructureSection load='1p74' size='340' side='right'caption='[[1p74]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1p74]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P74 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P74 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p74 OCA], [https://pdbe.org/1p74 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p74 RCSB], [https://www.ebi.ac.uk/pdbsum/1p74 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p74 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/AROE_HAEIN AROE_HAEIN]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p7/1p74_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p74 ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1P74 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Active as [http://en.wikipedia.org/wiki/Shikimate_dehydrogenase Shikimate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.25 1.1.1.25] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1P74 OCA].
*[[Shikimate dehydrogenase 3D structures|Shikimate dehydrogenase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
The crystal structure of shikimate dehydrogenase (AroE) reveals a unique NADPH binding mode., Ye S, Von Delft F, Brooun A, Knuth MW, Swanson RV, McRee DE, J Bacteriol. 2003 Jul;185(14):4144-51. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12837789 12837789]
[[Category: Haemophilus influenzae]]
[[Category: Haemophilus influenzae]]
[[Category: Shikimate dehydrogenase]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Brooun A]]
[[Category: Brooun, A.]]
[[Category: Knuth MW]]
[[Category: Delft, F.von.]]
[[Category: McRee DE]]
[[Category: Knuth, M.W.]]
[[Category: Swanson RV]]
[[Category: McRee, D.E.]]
[[Category: Ye S]]
[[Category: Swanson, R.V.]]
[[Category: Von Delft F]]
[[Category: Ye, S.]]
[[Category: haemophilus influenzae]]
[[Category: shikimate dehydrogenase]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:32:24 2007''

Latest revision as of 08:05, 14 February 2024

CRYSTAL STRUCTURE OF SHIKIMATE DEHYDROGENASE (AROE) FROM HAEMOPHILUS INFLUENZAE

1p74, resolution 2.40Å

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