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| [[Image:1prp.jpg|left|200px]]
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| {{Structure
| | ==CRYSTAL STRUCTURE OF D(CGCGAATTCGCG) COMPLEXED WITH PROPAMIDINE, A SHORT-CHAIN HOMOLOGUE OF THE DRUG PENTAMIDIN== |
| |PDB= 1prp |SIZE=350|CAPTION= <scene name='initialview01'>1prp</scene>, resolution 2.100Å
| | <StructureSection load='1prp' size='340' side='right'caption='[[1prp]], [[Resolution|resolution]] 2.10Å' scene=''> |
| |SITE=
| | == Structural highlights == |
| |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=TNT:1,3-BIS(AMIDINOPHENOXY)PROPANE'>TNT</scene>
| | <table><tr><td colspan='2'>[[1prp]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PRP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PRP FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| |GENE=
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TNT:1,3-BIS(AMIDINOPHENOXY)PROPANE'>TNT</scene></td></tr> |
| |DOMAIN=
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1prp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1prp OCA], [https://pdbe.org/1prp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1prp RCSB], [https://www.ebi.ac.uk/pdbsum/1prp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1prp ProSAT]</span></td></tr> |
| |RELATEDENTRY=
| | </table> |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1prp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1prp OCA], [http://www.ebi.ac.uk/pdbsum/1prp PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1prp RCSB]</span>
| | __TOC__ |
| }}
| | </StructureSection> |
| | | [[Category: Large Structures]] |
| '''CRYSTAL STRUCTURE OF D(CGCGAATTCGCG) COMPLEXED WITH PROPAMIDINE, A SHORT-CHAIN HOMOLOGUE OF THE DRUG PENTAMIDIN'''
| | [[Category: Jenkins TC]] |
| | | [[Category: Neidle S]] |
| | | [[Category: Nunn CM]] |
| ==Overview==
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| The crystal structure of the complex between propamidine and the self-complementary DNA dodecamer d(CGCGAATTCGCG) duplex has been determined to a resolution of 2.1 A and a R-factor of 17.4%. The propamidine binds to the DNA within the minor groove, with specific hydrogen-bonding interactions from the amidinium groups to the bases and sugar groups of the DNA, via a 4-base-pair binding site. A network of water molecules lies over the mouth of the minor groove. The structure provides a rationale for the superior DNA-binding properties of propamidine as compared to pentamidine [cf. Edwards, K. J., Jenkins, T. C. & Neidle, S. (1992) Biochemistry 31, 7104-7109]. This is seen to result from (i) decreased minor groove width at the binding site and (ii) reduced mobility of the bound propamidine molecule.
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| ==About this Structure==
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| 1PRP is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PRP OCA].
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| ==Reference==
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| Crystal structure of d(CGCGAATTCGCG) complexed with propamidine, a short-chain homologue of the drug pentamidine., Nunn CM, Jenkins TC, Neidle S, Biochemistry. 1993 Dec 21;32(50):13838-43. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8268158 8268158]
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| [[Category: Protein complex]] | |
| [[Category: Jenkins, T C.]] | |
| [[Category: Neidle, S.]] | |
| [[Category: Nunn, C M.]] | |
| [[Category: b-dna]]
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| [[Category: complexed with drug]]
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| [[Category: double helix]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:03:41 2008''
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