1ry9: Difference between revisions

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[[Image:1ry9.jpg|left|200px]]


{{Structure
==Spa15, a Type III Secretion Chaperone from Shigella flexneri==
|PDB= 1ry9 |SIZE=350|CAPTION= <scene name='initialview01'>1ry9</scene>, resolution 1.82&Aring;
<StructureSection load='1ry9' size='340' side='right'caption='[[1ry9]], [[Resolution|resolution]] 1.82&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=CL:CHLORIDE ION'>CL</scene>
<table><tr><td colspan='2'>[[1ry9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RY9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RY9 FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.82&#8491;</td></tr>
|GENE= SPAK, SPA15, CP0148 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=623 Shigella flexneri])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ry9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ry9 OCA], [https://pdbe.org/1ry9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ry9 RCSB], [https://www.ebi.ac.uk/pdbsum/1ry9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ry9 ProSAT]</span></td></tr>
 
</table>
'''Spa15, a Type III Secretion Chaperone from Shigella flexneri'''
== Function ==
 
[https://www.uniprot.org/uniprot/SPAK_SHIFL SPAK_SHIFL] Required for surface presentation of invasion plasmid antigens. Chaperone specialized in the storage of effectors within the bacterial cytoplasm, maintaining them in a secretion-competent state, and allowing their immediate delivery to target cells upon contact of the bacterium with the host cells. Has been shown to chaperone IpaA, IpgB1, OspC3 and probably also OspB.
 
== Evolutionary Conservation ==
==Overview==
[[Image:Consurf_key_small.gif|200px|right]]
Type III secretion (TTS) systems are used by many Gram-negative pathogens to inject virulence proteins into the cells of their hosts. Several of these virulence effectors require TTS chaperones that maintain them in a secretion-competent state. Whereas most chaperones bind only one effector, Spa15 from the human pathogen Shigella flexneri and homologous chaperones bind several seemingly unrelated effectors, and were proposed to form a special subgroup. Its 1.8 A crystal structure confirms this specific classification, showing that Spa15 has the same fold as other TTS effector chaperones, but forms a different dimer. The presence of hydrophobic sites on the Spa15 surface suggests that the different Spa15 effectors all possess similar structural elements that can bind these sites. Furthermore, the Spa15 structure reveals larger structural differences between class I chaperones than previously anticipated, which does not support the hypothesis that chaperone-effector complexes are structurally conserved and function as three-dimensional secretion signals.
Check<jmol>
 
  <jmolCheckbox>
==About this Structure==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ry/1ry9_consurf.spt"</scriptWhenChecked>
1RY9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RY9 OCA].  
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==Reference==
  </jmolCheckbox>
Structure of Spa15, a type III secretion chaperone from Shigella flexneri with broad specificity., van Eerde A, Hamiaux C, Perez J, Parsot C, Dijkstra BW, EMBO Rep. 2004 May;5(5):477-83. Epub 2004 Apr 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15088068 15088068]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ry9 ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Shigella flexneri]]
[[Category: Shigella flexneri]]
[[Category: Single protein]]
[[Category: Dijkstra BW]]
[[Category: Dijkstra, B W.]]
[[Category: Hamiaux C]]
[[Category: Eerde, A van.]]
[[Category: Parsot C]]
[[Category: Hamiaux, C.]]
[[Category: Perez J]]
[[Category: Parsot, C.]]
[[Category: Van Eerde A]]
[[Category: Perez, J.]]
[[Category: CL]]
[[Category: alpha/beta fold]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:57:51 2008''

Latest revision as of 08:26, 14 February 2024

Spa15, a Type III Secretion Chaperone from Shigella flexneri

1ry9, resolution 1.82Å

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