1s23: Difference between revisions

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[[Image:1s23.gif|left|200px]]


{{Structure
==Crystal Structure Analysis of the B-DNA Decamer CGCAATTGCG==
|PDB= 1s23 |SIZE=350|CAPTION= <scene name='initialview01'>1s23</scene>, resolution 1.60&Aring;
<StructureSection load='1s23' size='340' side='right'caption='[[1s23]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=CO:COBALT (II) ION'>CO</scene>
<table><tr><td colspan='2'>[[1s23]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S23 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S23 FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
|GENE=
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s23 OCA], [https://pdbe.org/1s23 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s23 RCSB], [https://www.ebi.ac.uk/pdbsum/1s23 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s23 ProSAT]</span></td></tr>
 
</table>
'''Crystal Structure Analysis of the B-DNA Decamer CGCAATTGCG'''
__TOC__
 
</StructureSection>
 
[[Category: Large Structures]]
==Overview==
[[Category: Murshudov GN]]
The deoxyoligonucleotide d(CGCAATTGCG) has previously been crystallized in four different space groups. The crystals diffract to moderate resolution (2.3-2.9 A). Here, a fifth crystal form that diffracts to higher resolution (1.6 A) is presented which was obtained thanks to the use of Co2+ and cryogenic temperatures. The availability of five different crystal structures allows a thorough analysis of the conformational variability of this DNA sequence. It is concluded that the central hexamer sequence CAATTG has a practically constant conformation under all conditions, whilst the terminal base pairs at both ends vary considerably as a result of differing interactions in the crystals. The new crystal structure presented here is stabilized by guanine-Co2+-guanine interactions and the formation of C1+ -G8.C3 triplexes between neighbouring duplexes. As a result of the higher resolution of the crystal structure, a more regular structure was obtained and a clear definition of the spine of hydration was observed which was not visible in the four previous structures.
[[Category: Steiner RA]]
 
[[Category: Subirana JA]]
==About this Structure==
[[Category: Valls N]]
1S23 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S23 OCA].  
[[Category: Wright G]]
 
==Reference==
DNA variability in five crystal structures of d(CGCAATTGCG)., Valls N, Wright G, Steiner RA, Murshudov GN, Subirana JA, Acta Crystallogr D Biol Crystallogr. 2004 Apr;60(Pt 4):680-5. Epub 2004, Mar 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15039556 15039556]
[[Category: Protein complex]]
[[Category: Murshudov, G N.]]
[[Category: Steiner, R A.]]
[[Category: Subirana, J A.]]
[[Category: Valls, N.]]
[[Category: Wright, G.]]
[[Category: CO]]
[[Category: cobalt]]
[[Category: deoxyoligonucleotide]]
[[Category: variability]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:59:25 2008''

Latest revision as of 08:27, 14 February 2024

Crystal Structure Analysis of the B-DNA Decamer CGCAATTGCG

1s23, resolution 1.60Å

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