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| [[Image:283d.jpg|left|200px]]<br /><applet load="283d" size="350" color="white" frame="true" align="right" spinBox="true"
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| caption="283d, resolution 2.300Å" />
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| '''A CURVED RNA HELIX INCORPORATING AN INTERNAL LOOP WITH G-A AND A-A NON-WATSON-CRICK BASE PAIRING'''<br />
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| ==Overview== | | ==A CURVED RNA HELIX INCORPORATING AN INTERNAL LOOP WITH G-A AND A-A NON-WATSON-CRICK BASE PAIRING== |
| The crystal structure of the RNA dodecamer 5'-GGCC(GAAA)GGCC-3' has been determined from x-ray diffraction data to 2.3-A resolution. In the crystal, these oligomers form double helices around twofold symmetry axes. Four consecutive non-Watson-Crick base pairs make up an internal loop in the middle of the duplex, including sheared G.A pairs and novel asymmetric A.A pairs. This internal loop sequence produces a significant curvature and narrowing of the double helix. The helix is curved by 34 degrees from end to end and the diameter is narrowed by 24% in the internal loop. A Mn2+ ion is bound directly to the N7 of the first guanine in the Watson-Crick region following the internal loop and the phosphate of the preceding residue. This Mn2+ location corresponds to a metal binding site observed in the hammerhead catalytic RNA.
| | <StructureSection load='283d' size='340' side='right'caption='[[283d]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | | == Structural highlights == |
| ==About this Structure== | | <table><tr><td colspan='2'>[[283d]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=283D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=283D FirstGlance]. <br> |
| 283D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MN:'>MN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=283D OCA].
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> |
| ==Reference==
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=283d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=283d OCA], [https://pdbe.org/283d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=283d RCSB], [https://www.ebi.ac.uk/pdbsum/283d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=283d ProSAT]</span></td></tr> |
| A curved RNA helix incorporating an internal loop with G.A and A.A non-Watson-Crick base pairing., Baeyens KJ, De Bondt HL, Pardi A, Holbrook SR, Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):12851-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8917508 8917508]
| | </table> |
| [[Category: ]] | | __TOC__ |
| [[Category: Protein complex]]
| | </StructureSection> |
| [[Category: Baeyens, K J.]]
| | [[Category: Large Structures]] |
| [[Category: Bondt, H L.De.]] | | [[Category: Baeyens KJ]] |
| [[Category: Holbrook, S R.]]
| | [[Category: De Bondt HL]] |
| [[Category: Pardi, A.]]
| | [[Category: Holbrook SR]] |
| [[Category: MN]]
| | [[Category: Pardi A]] |
| [[Category: curved]] | |
| [[Category: double helix]] | |
| [[Category: internal loop]] | |
| [[Category: mismatched]] | |
| [[Category: unusual rna]] | |
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:22:13 2008''
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