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| | ==CRYSTAL STRUCTURE OF THE DNA MODIFYING ENZYME BETA-GLUCOSYLTRANSFERASE IN THE PRESENCE AND ABSENCE OF THE SUBSTRATE URIDINE DIPHOSPHOGLUCOSE== |
| The line below this paragraph, containing "STRUCTURE_2bgt", creates the "Structure Box" on the page.
| | <StructureSection load='2bgt' size='340' side='right'caption='[[2bgt]], [[Resolution|resolution]] 2.20Å' scene=''> |
| You may change the PDB parameter (which sets the PDB file loaded into the applet)
| | == Structural highlights == |
| or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| | <table><tr><td colspan='2'>[[2bgt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BGT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BGT FirstGlance]. <br> |
| or leave the SCENE parameter empty for the default display.
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
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| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bgt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bgt OCA], [https://pdbe.org/2bgt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bgt RCSB], [https://www.ebi.ac.uk/pdbsum/2bgt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bgt ProSAT]</span></td></tr> |
| {{STRUCTURE_2bgt| PDB=2bgt | SCENE= }}
| | </table> |
| | | == Function == |
| '''CRYSTAL STRUCTURE OF THE DNA MODIFYING ENZYME BETA-GLUCOSYLTRANSFERASE IN THE PRESENCE AND ABSENCE OF THE SUBSTRATE URIDINE DIPHOSPHOGLUCOSE'''
| | [https://www.uniprot.org/uniprot/GSTB_BPT4 GSTB_BPT4] Catalyzes the transfer of glucose (Glc) from uridine diphosphoglucose (UDP-Glc) to 5-hydroxymethylcytosine (5-HMC) in double-stranded DNA. Is involved in a DNA modification process to protect the phage genome against its own nucleases and the host restriction endonuclease system. |
| | | __TOC__ |
| | | </StructureSection> |
| ==Overview== | | [[Category: Escherichia virus T4]] |
| Bacteriophage T4 beta-glucosyltransferase (EC 2.4.1.27) catalyses the transfer of glucose from uridine diphosphoglucose to hydroxymethyl groups of modified cytosine bases in T4 duplex DNA forming beta-glycosidic linkages. The enzyme forms part of a phage DNA protection system. We have solved and refined the crystal structure of recombinant beta-glucosyltransferase to 2.2 A resolution in the presence and absence of the substrate, uridine diphosphoglucose. The structure comprises two domains of similar topology, each reminiscent of a nucleotide binding fold. The two domains are separated by a central cleft which generates a concave surface along one side of the molecule. The substrate-bound complex reveals only clear electron density for the uridine diphosphate portion of the substrate. The UDPG is bound in a pocket at the bottom of the cleft between the two domains and makes extensive hydrogen bonding contacts with residues of the C-terminal domain only. The domains undergo a rigid body conformational change causing the structure to adopt a more closed conformation upon ligand binding. The movement of the domains is facilitated by a hinge region between residues 166 and 172. Electrostatic surface potential calculations reveal a large positive potential along the concave surface of the structure, suggesting a possible site for duplex DNA interaction.
| | [[Category: Large Structures]] |
| | | [[Category: Driessen HPC]] |
| ==About this Structure== | | [[Category: Freemont PS]] |
| 2BGT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BGT OCA].
| | [[Category: Rueger W]] |
| | | [[Category: Vrielink A]] |
| ==Reference== | |
| Crystal structure of the DNA modifying enzyme beta-glucosyltransferase in the presence and absence of the substrate uridine diphosphoglucose., Vrielink A, Ruger W, Driessen HP, Freemont PS, EMBO J. 1994 Aug 1;13(15):3413-22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8062817 8062817]
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| [[Category: DNA beta-glucosyltransferase]] | |
| [[Category: Enterobacteria phage t4]] | |
| [[Category: Single protein]] | |
| [[Category: Driessen, H P.C.]] | |
| [[Category: Freemont, P S.]] | |
| [[Category: Rueger, W.]] | |
| [[Category: Vrielink, A.]] | |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:16:02 2008''
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