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New page: left|200px<br /><applet load="2hj9" size="450" color="white" frame="true" align="right" spinBox="true" caption="2hj9, resolution 2.34Å" /> '''Crystal structure of...
 
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[[Image:2hj9.gif|left|200px]]<br /><applet load="2hj9" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2hj9, resolution 2.34&Aring;" />
'''Crystal structure of the Autoinducer-2-bound form of Vibrio harveyi LuxP complexed with the periplasmic domain of LuxQ'''<br />


==Overview==
==Crystal structure of the Autoinducer-2-bound form of Vibrio harveyi LuxP complexed with the periplasmic domain of LuxQ==
Bacteria sense their environment using receptors of the histidine sensor, kinase family, but how kinase activity is regulated by ligand binding is, not well understood. Autoinducer-2 (AI-2), a secreted signaling molecule, originally identified in studies of the marine bacterium Vibrio harveyi, regulates quorum-sensing responses and allows communication between, different bacterial species. AI-2 signal transduction in V. harveyi, requires the integral membrane receptor LuxPQ, comprised of periplasmic, binding protein (LuxP) and histidine sensor kinase (LuxQ) subunits., Combined X-ray crystallographic and functional studies show that AI-2, binding causes a major conformational change within LuxP, which in turn, stabilizes a quaternary arrangement in which two LuxPQ monomers are, asymmetrically associated. We propose that formation of this asymmetric, quaternary structure is responsible for repressing the kinase activity of, both LuxQ subunits and triggering the transition of V. harveyi into, quorum-sensing mode.
<StructureSection load='2hj9' size='340' side='right'caption='[[2hj9]], [[Resolution|resolution]] 2.34&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2hj9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_harveyi Vibrio harveyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HJ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HJ9 FirstGlance]. <br>
2HJ9 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Vibrio_harveyi Vibrio harveyi] with AI2 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2HJ9 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.34&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AI2:3A-METHYL-5,6-DIHYDRO-FURO[2,3-D][1,3,2]DIOXABOROLE-2,2,6,6A-TETRAOL'>AI2</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hj9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hj9 OCA], [https://pdbe.org/2hj9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hj9 RCSB], [https://www.ebi.ac.uk/pdbsum/2hj9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hj9 ProSAT]</span></td></tr>
Ligand-induced asymmetry in histidine sensor kinase complex regulates quorum sensing., Neiditch MB, Federle MJ, Pompeani AJ, Kelly RC, Swem DL, Jeffrey PD, Bassler BL, Hughson FM, Cell. 2006 Sep 22;126(6):1095-108. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16990134 16990134]
</table>
[[Category: Protein complex]]
== Function ==
[https://www.uniprot.org/uniprot/LUXP_VIBHA LUXP_VIBHA] Binds to the signaling molecule autoinducer 2 (AI-2), a furanosyl borate diester, (3a-methyl-5,6-dihydrofuro-[2,3d][1,3,2]dioxaborole-2,2,6,6a-tetraol). This complex then interacts with the LuxQ sensor protein.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hj/2hj9_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hj9 ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Vibrio harveyi]]
[[Category: Vibrio harveyi]]
[[Category: Hughson, F.M.]]
[[Category: Hughson FM]]
[[Category: Neiditch, M.B.]]
[[Category: Neiditch MB]]
[[Category: AI2]]
[[Category: autoinducer-2 (ai-2)]]
[[Category: histidine sensor kinase]]
[[Category: per/arnt/simple-minded (pas) fold]]
[[Category: periplasmic binding protein]]
[[Category: quorum sensing]]
 
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