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[[Image:2p87.gif|left|200px]]


{{Structure
==Crystal structure of the C-terminal domain of C. elegans pre-mRNA splicing factor Prp8==
|PDB= 2p87 |SIZE=350|CAPTION= <scene name='initialview01'>2p87</scene>, resolution 2.300&Aring;
<StructureSection load='2p87' size='340' side='right'caption='[[2p87]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[2p87]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P87 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P87 FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
|GENE= prp-8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 Caenorhabditis elegans])
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p87 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p87 OCA], [https://pdbe.org/2p87 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p87 RCSB], [https://www.ebi.ac.uk/pdbsum/2p87 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p87 ProSAT]</span></td></tr>
|DOMAIN=
</table>
|RELATEDENTRY=
== Function ==
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2p87 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p87 OCA], [http://www.ebi.ac.uk/pdbsum/2p87 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2p87 RCSB]</span>
[https://www.uniprot.org/uniprot/PRP8_CAEEL PRP8_CAEEL] Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex. Functions as scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site (By similarity).
}}
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p8/2p87_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2p87 ConSurf].
<div style="clear:both"></div>


'''Crystal structure of the C-terminal domain of C. elegans pre-mRNA splicing factor Prp8'''
==See Also==
 
*[[Pre-mRNA splicing factors 3D structures|Pre-mRNA splicing factors 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
Prp8 is a critical pre-mRNA splicing factor. Prp8 is proposed to help form and stabilize the spliceosome catalytic core and to be an important regulator of spliceosome activation. Mutations in human Prp8 (hPrp8) cause a severe form of the genetic disorder retinitis pigmentosa, RP13. Understanding the molecular mechanism of Prp8's function in pre-mRNA splicing and RP13 has been hindered by its large size (over 2000 amino acids) and remarkably low-sequence similarity with other proteins. Here we present the crystal structure of the C-terminal domain (the last 273 residues) of Caenorhabditis elegans Prp8 (cPrp8). The core of the C-terminal domain is an alpha/beta structure that forms the MPN (Mpr1, Pad1 N-terminal) fold but without Zn(2+) coordination. We propose that the C-terminal domain is a protein interaction domain instead of a Zn(2+)-dependent metalloenzyme as proposed for some MPN proteins. Mapping of RP13 mutants on the Prp8 structure suggests that these residues constitute a binding surface between Prp8 and other partner(s), and the disruption of this interaction provides a plausible molecular mechanism for RP13.
 
==About this Structure==
2P87 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P87 OCA].
 
==Reference==
Crystal structure of the C-terminal domain of splicing factor Prp8 carrying retinitis pigmentosa mutants., Zhang L, Shen J, Guarnieri MT, Heroux A, Yang K, Zhao R, Protein Sci. 2007 Jun;16(6):1024-31. Epub 2007 May 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17473007 17473007]
[[Category: Caenorhabditis elegans]]
[[Category: Caenorhabditis elegans]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Guarnieri, M T.]]
[[Category: Guarnieri MT]]
[[Category: Heroux, A.]]
[[Category: Heroux A]]
[[Category: Shen, J.]]
[[Category: Shen J]]
[[Category: Yang, K.]]
[[Category: Yang K]]
[[Category: Zhang, L.]]
[[Category: Zhang L]]
[[Category: Zhao, R.]]
[[Category: Zhao R]]
[[Category: alpha-beta]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:32:03 2008''

Latest revision as of 09:08, 21 February 2024

Crystal structure of the C-terminal domain of C. elegans pre-mRNA splicing factor Prp8

2p87, resolution 2.30Å

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