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| | ==Characterization of a trifunctional mimivirus mRNA capping enzyme and crystal structure of the RNA triphosphatase domainm.== |
| The line below this paragraph, containing "STRUCTURE_2qy2", creates the "Structure Box" on the page.
| | <StructureSection load='2qy2' size='340' side='right'caption='[[2qy2]], [[Resolution|resolution]] 2.00Å' scene=''> |
| You may change the PDB parameter (which sets the PDB file loaded into the applet)
| | == Structural highlights == |
| or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| | <table><tr><td colspan='2'>[[2qy2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mimivirus Mimivirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QY2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QY2 FirstGlance]. <br> |
| or leave the SCENE parameter empty for the default display.
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
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| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr> |
| {{STRUCTURE_2qy2| PDB=2qy2 | SCENE= }}
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qy2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qy2 OCA], [https://pdbe.org/2qy2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qy2 RCSB], [https://www.ebi.ac.uk/pdbsum/2qy2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qy2 ProSAT]</span></td></tr> |
| | | </table> |
| '''Characterization of a trifunctional mimivirus mRNA capping enzyme and crystal structure of the RNA triphosphatase domainm.'''
| | == Function == |
| | | [https://www.uniprot.org/uniprot/MCE_MIMIV MCE_MIMIV] Responsible for methylating the 5'-cap structure of mRNAs. |
| | | __TOC__ |
| ==Overview== | | </StructureSection> |
| The RNA triphosphatase (RTPase) components of the mRNA capping apparatus are a bellwether of eukaryal taxonomy. Fungal and protozoal RTPases belong to the triphosphate tunnel metalloenzyme (TTM) family, exemplified by yeast Cet1. Several large DNA viruses encode metal-dependent RTPases unrelated to the cysteinyl-phosphatase RTPases of their metazoan host organisms. The origins of DNA virus RTPases are unclear because they are structurally uncharacterized. Mimivirus, a giant virus of amoeba, resembles poxviruses in having a trifunctional capping enzyme composed of a metal-dependent RTPase module fused to guanylyltransferase (GTase) and guanine-N7 methyltransferase domains. The crystal structure of mimivirus RTPase reveals a minimized tunnel fold and an active site strikingly similar to that of Cet1. Unlike homodimeric fungal RTPases, mimivirus RTPase is a monomer. The mimivirus TTM-type RTPase-GTase fusion resembles the capping enzymes of amoebae, providing evidence that the ancestral large DNA virus acquired its capping enzyme from a unicellular host.
| | [[Category: Large Structures]] |
| | |
| ==About this Structure== | |
| 2QY2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mimivirus Mimivirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QY2 OCA].
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| ==Reference== | |
| Characterization of a trifunctional mimivirus mRNA capping enzyme and crystal structure of the RNA triphosphatase domain., Benarroch D, Smith P, Shuman S, Structure. 2008 Apr;16(4):501-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18400173 18400173]
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| [[Category: Mimivirus]] | | [[Category: Mimivirus]] |
| [[Category: Single protein]]
| | [[Category: Benarroch D]] |
| [[Category: Benarroch, D.]] | | [[Category: Shuman S]] |
| [[Category: Shuman, S.]] | | [[Category: Smith P]] |
| [[Category: Smith, P.]] | |
| [[Category: Beta tunnel]]
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| [[Category: Hydrolase]]
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| [[Category: Mrna capping]]
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| [[Category: Mrna processing]]
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| [[Category: Multifunctional enzyme]]
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| [[Category: Nucleotidyltransferase]]
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| [[Category: Phosphatase]]
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| [[Category: S-adenosyl-l-methionine]]
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| [[Category: Transferase]]
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| [[Category: Viral protein]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 24 09:27:55 2008''
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