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[[Image:2ree.jpg|left|200px]]<br /><applet load="2ree" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2ree, resolution 1.950&Aring;" />
'''Crystal structure of the loading GNATL domain of CurA from Lyngbya majuscula'''<br />


==Overview==
==Crystal structure of the loading GNATL domain of CurA from Lyngbya majuscula==
An unexpected biochemical strategy for chain initiation is described for the loading module of the polyketide synthase of curacin A, an anticancer lead derived from the marine cyanobacterium Lyngbya majuscula. A central GCN5-related N-acetyltransferase (GNAT) domain bears bifunctional decarboxylase/S-acetyltransferase activity, both unprecedented for the GNAT superfamily. A CurA loading tridomain, consisting of an adaptor domain, the GNAT domain, and an acyl carrier protein, was assessed biochemically, revealing that a domain showing homology to GNAT (GNAT(L)) catalyzes (i) decarboxylation of malonyl-coenzyme A (malonyl-CoA) to acetyl-CoA and (ii) direct S-acetyl transfer from acetyl-CoA to load an adjacent acyl carrier protein domain (ACP(L)). Moreover, the N-terminal adapter domain was shown to facilitate acetyl-group transfer. Crystal structures of GNAT(L) were solved at 1.95 angstroms (ligand-free form) and 2.75 angstroms (acyl-CoA complex), showing distinct substrate tunnels for acyl-CoA and holo-ACP(L) binding. Modeling and site-directed mutagenesis experiments demonstrated that histidine-389 and threonine-355, at the convergence of the CoA and ACP tunnels, participate in malonyl-CoA decarboxylation but not in acetyl-group transfer. Decarboxylation precedes acetyl-group transfer, leading to acetyl-ACP(L) as the key curacin A starter unit.
<StructureSection load='2ree' size='340' side='right'caption='[[2ree]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2ree]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Moorena_producens_19L Moorena producens 19L]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2REE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2REE FirstGlance]. <br>
2REE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lyngbya_majuscula Lyngbya majuscula] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2REE OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ree FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ree OCA], [https://pdbe.org/2ree PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ree RCSB], [https://www.ebi.ac.uk/pdbsum/2ree PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ree ProSAT]</span></td></tr>
GNAT-like strategy for polyketide chain initiation., Gu L, Geders TW, Wang B, Gerwick WH, Hakansson K, Smith JL, Sherman DH, Science. 2007 Nov 9;318(5852):970-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17991863 17991863]
</table>
[[Category: Lyngbya majuscula]]
== Function ==
[[Category: Single protein]]
[https://www.uniprot.org/uniprot/Q6DNF2_9CYAN Q6DNF2_9CYAN]  
[[Category: Geders, T W.]]
== Evolutionary Conservation ==
[[Category: Smith, J L.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: CL]]
Check<jmol>
[[Category: GOL]]
  <jmolCheckbox>
[[Category: SO4]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/re/2ree_consurf.spt"</scriptWhenChecked>
[[Category: curacin]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: decarboxylase]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: gnat]]
  </jmolCheckbox>
[[Category: loading]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ree ConSurf].
[[Category: lyase]]
<div style="clear:both"></div>
[[Category: phosphopantetheine]]
__TOC__
[[Category: polyketide synthase]]
</StructureSection>
[[Category: s-acetyltransferase]]
[[Category: Large Structures]]
[[Category: transferase]]
[[Category: Moorena producens 19L]]
 
[[Category: Geders TW]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:46:37 2008''
[[Category: Smith JL]]
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