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| [[Image:377d.gif|left|200px]]<br /><applet load="377d" size="350" color="white" frame="true" align="right" spinBox="true"
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| caption="377d, resolution 1.760Å" />
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| '''5'-R(*CP*GP*UP*AP*CP*G)-3''''<br />
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| ==Overview== | | ==5'-R(*CP*GP*UP*AP*CP*DG)-3'== |
| The crystal structure of the alternating RNA r(CGUAC)dG with a 3'-terminal deoxy G residue has been determined at 1.76 A resolution. The crystal belongs to the orthorhombic space group C2221, unit-cell dimensions a = 29.53, b = 44.61 and c = 94.18 A, with two independent duplexes (I and II) per asymmetric unit. The structure was solved by the molecular-replacement method. The final R factor was 18.8% using 4757 reflections in the resolution range 8.0-1.76 A. The model contains a total of 496 atoms and 85 solvent molecules. The two duplexes form the repeating unit and stack in the usual head-to-tail (5',3'/5',3') fashion into a pseudocontinuous helical column. Almost all of the 2'-hydroxyl groups are engaged in the three modes of water-mediated interactions to the base N3/O2 atoms, the sugar O4' atoms and the backbone phosphates. Thus, the 2'-hydroxyl group of RNA is probably contributing to the stability of the duplexes.
| | <StructureSection load='377d' size='340' side='right'caption='[[377d]], [[Resolution|resolution]] 1.76Å' scene=''> |
| | | == Structural highlights == |
| ==About this Structure== | | <table><tr><td colspan='2'>[[377d]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=377D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=377D FirstGlance]. <br> |
| 377D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=377D OCA].
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.76Å</td></tr> |
| | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=377d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=377d OCA], [https://pdbe.org/377d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=377d RCSB], [https://www.ebi.ac.uk/pdbsum/377d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=377d ProSAT]</span></td></tr> |
| ==Reference== | | </table> |
| 1.76 A structure of a pyrimidine start alternating A-RNA hexamer r(CGUAC)dG., Biswas R, Mitra SN, Sundaralingam M, Acta Crystallogr D Biol Crystallogr. 1998 Jul 1;54(Pt 4):570-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9761851 9761851] | | __TOC__ |
| [[Category: Protein complex]] | | </StructureSection> |
| [[Category: Biswas, R.]]
| | [[Category: Large Structures]] |
| [[Category: Mitra, S N.]] | | [[Category: Biswas R]] |
| [[Category: Sundaralingam, M.]] | | [[Category: Mitra SN]] |
| [[Category: a-rna]] | | [[Category: Sundaralingam M]] |
| [[Category: double helix]] | |
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:02:28 2008''
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