4sli: Difference between revisions

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[[Image:4sli.jpg|left|200px]]


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==LEECH INTRAMOLECULAR TRANS-SIALIDASE COMPLEXED WITH 2-PROPENYL-NEU5AC, AN INACTIVE SUBSTRATE ANALOGUE==
The line below this paragraph, containing "STRUCTURE_4sli", creates the "Structure Box" on the page.
<StructureSection load='4sli' size='340' side='right'caption='[[4sli]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[4sli]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Macrobdella_decora Macrobdella decora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4SLI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4SLI FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
-->
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CNP:2-PROPENYL-N-ACETYL-NEURAMIC+ACID'>CNP</scene></td></tr>
{{STRUCTURE_4sli|  PDB=4sli  |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4sli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4sli OCA], [https://pdbe.org/4sli PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4sli RCSB], [https://www.ebi.ac.uk/pdbsum/4sli PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4sli ProSAT]</span></td></tr>
 
</table>
'''LEECH INTRAMOLECULAR TRANS-SIALIDASE COMPLEXED WITH 2-PROPENYL-NEU5AC, AN INACTIVE SUBSTRATE ANALOGUE'''
== Function ==
 
[https://www.uniprot.org/uniprot/NANL_MACDE NANL_MACDE]
 
== Evolutionary Conservation ==
==Overview==
[[Image:Consurf_key_small.gif|200px|right]]
Intramolecular trans-sialidase from leech (Macrobdella decora) is the first member of the sialidase superfamily found to exhibit strict specificity towards the cleavage of terminal Neu5Acalpha2--&gt;3Gal linkage in sialoglycoconjugates. Its release of 2,7-anhydro-Neu5Ac instead of Neu5Ac indicates that it catalyzes an intramolecular trans-sialosyl reaction. Crystal structures of its complexes with an inactive substrate analogue 2-propenyl-Neu5Ac, and with the product 2,7-anhydro-Neu5Ac, have been determined to 1.8 A resolution. The boat conformation of the pyranose observed in the complexes supports the proposed enzymatic mechanism that O7 of an axial 6-glycerol group attacks the positively charged C2 of the intermediate. A generalized mechanism is proposed for the sialidase superfamily.
Check<jmol>
 
  <jmolCheckbox>
==About this Structure==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sl/4sli_consurf.spt"</scriptWhenChecked>
4SLI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Macrobdella_decora Macrobdella decora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4SLI OCA].  
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==Reference==
  </jmolCheckbox>
The 1.8 A structures of leech intramolecular trans-sialidase complexes: evidence of its enzymatic mechanism., Luo Y, Li SC, Li YT, Luo M, J Mol Biol. 1999 Jan 8;285(1):323-32. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9878409 9878409]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=4sli ConSurf].
[[Category: Exo-alpha-sialidase]]
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Macrobdella decora]]
[[Category: Macrobdella decora]]
[[Category: Single protein]]
[[Category: Li SC]]
[[Category: Li, S C.]]
[[Category: Li YT]]
[[Category: Li, Y T.]]
[[Category: Luo M]]
[[Category: Luo, M.]]
[[Category: Luo Y]]
[[Category: Luo, Y.]]
[[Category: Enzyme]]
[[Category: Intramolecular trans-sialidase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 22:29:33 2008''
Retrieved from "https://proteopedia.org/4sli"