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==Crystal structure of tRNA dependent lantibiotic dehydratase MibB from Microbispora sp. 107891==
==Crystal structure of tRNA dependent lantibiotic dehydratase MibB from Microbispora sp. 107891==
<StructureSection load='5ehk' size='340' side='right' caption='[[5ehk]], [[Resolution|resolution]] 2.71&Aring;' scene=''>
<StructureSection load='5ehk' size='340' side='right'caption='[[5ehk]], [[Resolution|resolution]] 2.71&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5ehk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_baa-20 Atcc baa-20]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EHK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EHK FirstGlance]. <br>
<table><tr><td colspan='2'>[[5ehk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Microbispora_corallina Microbispora corallina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EHK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EHK FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mibB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83302 ATCC BAA-20])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.708&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ehk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ehk OCA], [http://pdbe.org/5ehk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ehk RCSB], [http://www.ebi.ac.uk/pdbsum/5ehk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ehk ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ehk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ehk OCA], [https://pdbe.org/5ehk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ehk RCSB], [https://www.ebi.ac.uk/pdbsum/5ehk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ehk ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/E2IHB7_9ACTN E2IHB7_9ACTN]
Class I lantibiotic dehydratases dehydrate selected Ser/Thr residues of a precursor peptide. Recent studies demonstrated the requirement of glutamyl-tRNAGlu for Ser/Thr activation by one of these enzymes (NisB) from the Firmicute Lactococcus lactis. However, the generality of glutamyl-tRNAGlu usage and the tRNA specificity of lantibiotic dehydratases have not been established. Here we report the 2.7-A resolution crystal structure, along with the glutamyl-tRNAGlu utilization of MibB, a lantibiotic dehydratase from the Actinobacterium Microbispora sp. 107891 involved in the biosynthesis of the clinical candidate NAI-107. Biochemical assays revealed nucleotides A73 and U72 within the tRNAGlu acceptor stem to be important for MibB glutamyl-tRNAGlu usage. Using this knowledge, an expression system for the production of NAI-107 analogs in Escherichia coli was developed, overcoming the inability of MibB to utilize E. coli tRNAGlu. Our work provides evidence for a common tRNAGlu-dependent dehydration mechanism, paving the way for the characterization of lantibiotics from various phyla.
 
Structure and tRNA Specificity of MibB, a Lantibiotic Dehydratase from Actinobacteria Involved in NAI-107 Biosynthesis.,Ortega MA, Hao Y, Walker MC, Donadio S, Sosio M, Nair SK, van der Donk WA Cell Chem Biol. 2016 Feb 8. pii: S2451-9456(16)30001-0. doi:, 10.1016/j.chembiol.2015.11.017. PMID:26877024<ref>PMID:26877024</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5ehk" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Atcc baa-20]]
[[Category: Large Structures]]
[[Category: Hao, Y]]
[[Category: Microbispora corallina]]
[[Category: Nair, S K]]
[[Category: Hao Y]]
[[Category: Hydrolase]]
[[Category: Nair SK]]
[[Category: Lantibiotic dehydratase]]
[[Category: Microbispora sp. 107891]]
[[Category: Nai-107]]
[[Category: Trna dependent]]
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