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New page: left|200px<br /><applet load="3bk6" size="350" color="white" frame="true" align="right" spinBox="true" caption="3bk6, resolution 3.20Å" /> '''Crystal structure of...
 
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[[Image:3bk6.jpg|left|200px]]<br /><applet load="3bk6" size="350" color="white" frame="true" align="right" spinBox="true"
caption="3bk6, resolution 3.20&Aring;" />
'''Crystal structure of a core domain of stomatin from Pyrococcus horikoshii'''<br />


==Overview==
==Crystal structure of a core domain of stomatin from Pyrococcus horikoshii==
Stomatin is a major integral membrane protein of human erythrocytes, the absence of which is associated with a form of hemolytic anemia known as hereditary stomatocytosis. However, the function of stomatin is not fully understood. An open reading frame, PH1511, from the hyperthermophilic archaeon Pyrococcus horikoshii encodes p-stomatin, a prokaryotic stomatin. Here, we report the first crystal structure of a stomatin ortholog, the core domain of the p-stomatin PH1511p (residues 56-234 of PH1511p, designated as PhSto(CD)). PhSto(CD) forms a novel homotrimeric structure. Three alpha/beta domains form a triangle of about 50 A on each side, and three alpha-helical segments of about 60 A in length extend from the apexes of the triangle. The alpha/beta domain of PhSto(CD) is partly similar in structure to the band-7 domain of mouse flotillin-2. While the alpha/beta domain is relatively rigid, the alpha-helical segment shows conformational flexibility, adapting to the neighboring environment. One alpha-helical segment forms an anti-parallel coiled coil with another alpha-helical segment from a symmetry-related molecule. The alpha-helical segment shows a heptad repeat pattern, and mainly hydrophobic residues form a coiled-coil interface. According to chemical cross-linking experiments, PhSto(CD) would be able to assemble into an oligomeric form. The coiled-coil fold observed in the crystal probably contributes to self-association.
<StructureSection load='3bk6' size='340' side='right'caption='[[3bk6]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[3bk6]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BK6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BK6 FirstGlance]. <br>
3BK6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BK6 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bk6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bk6 OCA], [https://pdbe.org/3bk6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bk6 RCSB], [https://www.ebi.ac.uk/pdbsum/3bk6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bk6 ProSAT]</span></td></tr>
==Reference==
</table>
Crystal structure of a core domain of stomatin from Pyrococcus horikoshii Illustrates a novel trimeric and coiled-coil fold., Yokoyama H, Fujii S, Matsui I, J Mol Biol. 2008 Feb 22;376(3):868-78. Epub 2008 Jan 8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18182167 18182167]
== Function ==
[https://www.uniprot.org/uniprot/PSTOM_PYRHO PSTOM_PYRHO]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bk/3bk6_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bk6 ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pyrococcus horikoshii]]
[[Category: Pyrococcus horikoshii]]
[[Category: Single protein]]
[[Category: Fujii S]]
[[Category: Fujii, S.]]
[[Category: Matsui I]]
[[Category: Matsui, I.]]
[[Category: Yokoyama H]]
[[Category: Yokoyama, H.]]
[[Category: archaea]]
[[Category: coiled-coil]]
[[Category: flotillin]]
[[Category: membrane fusion]]
[[Category: membrane protein]]
[[Category: pyrococcus horikoshii]]
[[Category: spfh]]
[[Category: stomatin]]
[[Category: trafficking]]
[[Category: transmembrane]]
[[Category: trimer]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:06:19 2008''

Latest revision as of 14:03, 13 March 2024

Crystal structure of a core domain of stomatin from Pyrococcus horikoshii

3bk6, resolution 3.20Å

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