5w8d: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
m Protected "5w8d" [edit=sysop:move=sysop]
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:


==The structure of a COA-dependent acyl-homoserine lactone synthase, BjaI, with MTA==
==The structure of a COA-dependent acyl-homoserine lactone synthase, BjaI, with MTA==
<StructureSection load='5w8d' size='340' side='right' caption='[[5w8d]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
<StructureSection load='5w8d' size='340' side='right'caption='[[5w8d]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5w8d]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W8D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W8D FirstGlance]. <br>
<table><tr><td colspan='2'>[[5w8d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bradyrhizobium_japonicum Bradyrhizobium japonicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W8D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5W8D FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MTA:5-DEOXY-5-METHYLTHIOADENOSINE'>MTA</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w8d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w8d OCA], [http://pdbe.org/5w8d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w8d RCSB], [http://www.ebi.ac.uk/pdbsum/5w8d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w8d ProSAT]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MTA:5-DEOXY-5-METHYLTHIOADENOSINE'>MTA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5w8d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w8d OCA], [https://pdbe.org/5w8d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5w8d RCSB], [https://www.ebi.ac.uk/pdbsum/5w8d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5w8d ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In several Proteobacteria, LuxI-type enzymes catalyze the biosynthesis of acyl-homoserine lactones (AHL) signals using S-adenosyl-l-methionine and either cellular acyl carrier protein (ACP)-coupled fatty acids or CoA-aryl/acyl moieties as progenitors. Little is known about the molecular mechanism of signal biosynthesis, the basis for substrate specificity, or the rationale for donor specificity for any LuxI member. Here, we present several cocrystal structures of BjaI, a CoA-dependent LuxI homolog that represent views of enzyme complexes that exist along the reaction coordinate of signal synthesis. Complementary biophysical, structure-function, and kinetic analysis define the features that facilitate the unusual acyl conjugation with S-adenosylmethionine (SAM). We also identify the determinant that establishes specificity for the acyl donor and identify residues that are critical for acyl/aryl specificity. These results highlight how a prevalent scaffold has evolved to catalyze quorum signal synthesis and provide a framework for the design of small-molecule antagonists of quorum signaling.
Molecular basis for the substrate specificity of quorum signal synthases.,Dong SH, Frane ND, Christensen QH, Greenberg EP, Nagarajan R, Nair SK Proc Natl Acad Sci U S A. 2017 Aug 22;114(34):9092-9097. doi:, 10.1073/pnas.1705400114. Epub 2017 Aug 7. PMID:28784791<ref>PMID:28784791</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5w8d" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Dong, S H]]
[[Category: Bradyrhizobium japonicum]]
[[Category: Nair, S K]]
[[Category: Large Structures]]
[[Category: Acyl-homoserine lactone]]
[[Category: Dong S-H]]
[[Category: Biosynthetic protein]]
[[Category: Nair SK]]
[[Category: Bjai]]
[[Category: Coenzyme some]]

Latest revision as of 14:14, 13 March 2024

The structure of a COA-dependent acyl-homoserine lactone synthase, BjaI, with MTA

5w8d, resolution 2.00Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=5w8d&oldid=4090149"