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==Trans-acting transferase from Disorazole synthase in complex with Acetate==
==Trans-acting transferase from Disorazole synthase in complex with Acetate==
<StructureSection load='3sbm' size='340' side='right' caption='[[3sbm]], [[Resolution|resolution]] 1.35&Aring;' scene=''>
<StructureSection load='3sbm' size='340' side='right'caption='[[3sbm]], [[Resolution|resolution]] 1.35&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3sbm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sorangium_cellulosum Sorangium cellulosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SBM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SBM FirstGlance]. <br>
<table><tr><td colspan='2'>[[3sbm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sorangium_cellulosum Sorangium cellulosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SBM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SBM FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene><br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.35&#8491;</td></tr>
<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">dszD, disD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=56 Sorangium cellulosum])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene></td></tr>
<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/[Acyl-carrier-protein]_S-malonyltransferase [Acyl-carrier-protein] S-malonyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.39 2.3.1.39] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sbm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sbm OCA], [https://pdbe.org/3sbm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sbm RCSB], [https://www.ebi.ac.uk/pdbsum/3sbm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sbm ProSAT]</span></td></tr>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sbm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sbm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3sbm RCSB], [http://www.ebi.ac.uk/pdbsum/3sbm PDBsum]</span></td></tr>
</table>
<table>
== Function ==
<div style="background-color:#fffaf0;">
[https://www.uniprot.org/uniprot/Q4U443_SORCE Q4U443_SORCE]
== Publication Abstract from PubMed ==
The 1.51 A resolution X-ray crystal structure of the trans-acyltransferase (AT) from the "AT-less" disorazole synthase (DSZS) and that of its acetate complex at 1.35 A resolution are reported. Separately, comprehensive alanine-scanning mutagenesis of one of its acyl carrier protein substrates (ACP1 from DSZS) led to the identification of a conserved Asp45 residue on the ACP, which contributes to the substrate specificity of this unusual enzyme. Together, these experimental findings were used to derive a model for the selective association of the DSZS AT and its ACP substrate. With a goal of structurally characterizing the AT-ACP interface, a strategy was developed for covalently cross-linking the active site Ser --&gt; Cys mutant of the DSZS AT to its ACP substrate and for purifying the resulting AT-ACP complex to homogeneity. The S86C DSZS AT mutant was found to be functional, albeit with a transacylation efficiency 200-fold lower than that of its wild-type counterpart. Our findings provide new insights as well as new opportunities for high-resolution analysis of an important protein-protein interface in polyketide synthases.
 
Structure and Mechanism of the trans-Acting Acyltransferase from the Disorazole Synthase.,Wong FT, Jin X, Mathews II, Cane DE, Khosla C Biochemistry. 2011 Jul 5. PMID:21707057<ref>PMID:21707057</ref>
 
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
</div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Sorangium cellulosum]]
[[Category: Sorangium cellulosum]]
[[Category: Jin, X.]]
[[Category: Jin X]]
[[Category: Khosla, C.]]
[[Category: Khosla C]]
[[Category: Mathews, I I.]]
[[Category: Mathews II]]
[[Category: Wong, F T.]]
[[Category: Wong FT]]
[[Category: Transferase]]

Latest revision as of 12:52, 14 March 2024

Trans-acting transferase from Disorazole synthase in complex with Acetate

3sbm, resolution 1.35Å

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