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| ==Crystal Structure of Protein Ms6760 from Mycobacterium smegmatis== | | ==Crystal Structure of Protein Ms6760 from Mycobacterium smegmatis== |
| <StructureSection load='3uid' size='340' side='right' caption='[[3uid]], [[Resolution|resolution]] 1.57Å' scene=''> | | <StructureSection load='3uid' size='340' side='right'caption='[[3uid]], [[Resolution|resolution]] 1.57Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[3uid]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycs2 Mycs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UID OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UID FirstGlance]. <br> | | <table><tr><td colspan='2'>[[3uid]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UID OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UID FirstGlance]. <br> |
| </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MSMEG_6760 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=246196 MYCS2])</td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.571Å</td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uid FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uid OCA], [http://pdbe.org/3uid PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3uid RCSB], [http://www.ebi.ac.uk/pdbsum/3uid PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3uid ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uid FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uid OCA], [https://pdbe.org/3uid PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uid RCSB], [https://www.ebi.ac.uk/pdbsum/3uid PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uid ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [https://www.uniprot.org/uniprot/A0R731_MYCS2 A0R731_MYCS2] |
| The structure of Msmeg_6760, a protein of unknown function, has been determined. Biochemical and bioinformatics analyses determined that Msmeg_6760 interacts with a protein encoded in the same operon, Msmeg_6762, and predicted that the operon is a toxin-antitoxin (TA) system. Structural comparison of Msmeg_6760 with proteins of known function suggests that Msmeg_6760 binds a hydrophobic ligand in a buried cavity lined by large hydrophobic residues. Access to this cavity could be controlled by a gate-latch mechanism. The function of the Msmeg_6760 toxin is unknown, but structure-based predictions revealed that Msmeg_6760 and Msmeg_6762 are homologous to Rv2034 and Rv2035, a predicted novel TA system involved in Mycobacterium tuberculosis latency during macrophage infection. The Msmeg_6760 toxin fold has not been previously described for bacterial toxins and its unique structural features suggest that toxin activation is likely to be mediated by a novel mechanism.
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| Crystal structure of the toxin Msmeg_6760, the structural homolog of Mycobacterium tuberculosis Rv2035, a novel type II toxin involved in the hypoxic response.,Bajaj RA, Arbing MA, Shin A, Cascio D, Miallau L Acta Crystallogr F Struct Biol Commun. 2016 Dec 1;72(Pt 12):863-869. Epub 2016, Nov 19. PMID:27917833<ref>PMID:27917833</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 3uid" style="background-color:#fffaf0;"></div>
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| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Mycs2]] | | [[Category: Large Structures]] |
| [[Category: Arbing, M]] | | [[Category: Mycolicibacterium smegmatis MC2 155]] |
| [[Category: Bajaj, R A]] | | [[Category: Arbing M]] |
| [[Category: Cascio, D]] | | [[Category: Bajaj RA]] |
| [[Category: Eisenberg, D]] | | [[Category: Cascio D]] |
| [[Category: Miallau, L]] | | [[Category: Eisenberg D]] |
| [[Category: Structural genomic]]
| | [[Category: Miallau L]] |
| [[Category: Beta sandwich]]
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| [[Category: Srpbcc superfamily]]
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| [[Category: Tbsgc]]
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| [[Category: Uncharacterized protein]]
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| [[Category: Unknown function]]
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