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==X-ray crystal structure of Prostaglandin f synthase from Leishmania major Friedlin bound to NADPH==
==X-ray crystal structure of Prostaglandin f synthase from Leishmania major Friedlin bound to NADPH==
<StructureSection load='4g5d' size='340' side='right' caption='[[4g5d]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
<StructureSection load='4g5d' size='340' side='right'caption='[[4g5d]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4g5d]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Leishmania_major_mhom/il/81/friedlin Leishmania major mhom/il/81/friedlin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G5D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G5D FirstGlance]. <br>
<table><tr><td colspan='2'>[[4g5d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Leishmania_major_strain_Friedlin Leishmania major strain Friedlin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G5D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4G5D FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4f40|4f40]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PGFS, LMJF_31_2150 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=347515 Leishmania major MHOM/IL/81/Friedlin])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4g5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g5d OCA], [https://pdbe.org/4g5d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4g5d RCSB], [https://www.ebi.ac.uk/pdbsum/4g5d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4g5d ProSAT]</span></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Prostaglandin-F_synthase Prostaglandin-F synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.188 1.1.1.188] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g5d OCA], [http://pdbe.org/4g5d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4g5d RCSB], [http://www.ebi.ac.uk/pdbsum/4g5d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4g5d ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/PGFS_LEIMA PGFS_LEIMA]] Catalyzes the NADP-dependent formation of prostaglandin F2-alpha from prostaglandin H2. Has also aldo/ketoreductase activity toward the synthetic substrates 9,10-phenanthrenequinone and p-nitrobenzaldehyde.<ref>PMID:12633659</ref> 
[https://www.uniprot.org/uniprot/PGFS_LEIMA PGFS_LEIMA] Catalyzes the NADP-dependent formation of prostaglandin F2-alpha from prostaglandin H2. Has also aldo/ketoreductase activity toward the synthetic substrates 9,10-phenanthrenequinone and p-nitrobenzaldehyde.<ref>PMID:12633659</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structures of prostaglandin F synthase (PGF) from both Leishmania major and Trypanosoma cruzi with and without their cofactor NADP have been determined to resolutions of 2.6 A for T. cruzi PGF, 1.25 A for T. cruzi PGF with NADP, 1.6 A for L. major PGF and 1.8 A for L. major PGF with NADP. These structures were determined by molecular replacement to a final R factor of less than 18.6% (Rfree of less than 22.9%). PGF in the infectious protozoa L. major and T. cruzi is a potential therapeutic target.
 
Structures of prostaglandin F synthase from the protozoa Leishmania major and Trypanosoma cruzi with NADP.,Moen SO, Fairman JW, Barnes SR, Sullivan A, Nakazawa-Hewitt S, Van Voorhis WC, Staker BL, Lorimer DD, Myler PJ, Edwards TE Acta Crystallogr F Struct Biol Commun. 2015 May;71(Pt 5):609-14. doi:, 10.1107/S2053230X15006883. Epub 2015 Apr 21. PMID:25945716<ref>PMID:25945716</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4g5d" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Prostaglandin F synthase|Prostaglandin F synthase]]
*[[Prostaglandin F synthase 3D structures|Prostaglandin F synthase 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Leishmania major mhom/il/81/friedlin]]
[[Category: Large Structures]]
[[Category: Prostaglandin-F synthase]]
[[Category: Leishmania major strain Friedlin]]
[[Category: Structural genomic]]
[[Category: National institute of allergy and infectious disease]]
[[Category: Niaid]]
[[Category: Oxidoreductase]]
[[Category: Ssgcid]]
Retrieved from "https://proteopedia.org/4g5d"