8xms: Difference between revisions

Line 1:

~~'''Unreleased structure'''~~

~~The entry~~ 8xms is ~~ON HOLD until Paper Publication~~

==Crystal structure of Porcine Circovirus type II Rep ATPase domain==

~~Authors~~:

== Structural highlights ==

<table><tr><td colspan='2'>[[8xms]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Porcine_circovirus_2 Porcine circovirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8XMS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8XMS FirstGlance]. <br>

~~Description~~:

</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr>

[[Category: ~~Unreleased~~ Structures]]

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8xms FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8xms OCA], [https://pdbe.org/8xms PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8xms RCSB], [https://www.ebi.ac.uk/pdbsum/8xms PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8xms ProSAT]</span></td></tr>

</table>

== Function ==

[https://www.uniprot.org/uniprot/REP_PCV2 REP_PCV2] Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep and/or Rep' binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the conserved sequence 5'-AGTATTAC-3' in the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, and nucleotidyl transferase.<ref>PMID:12954212</ref> <ref>PMID:25768890</ref>

== References ==

__TOC__

</StructureSection>

[[Category: Large Structures]]

[[Category: Porcine circovirus 2]]

[[Category: Guan SY]]

[[Category: Song YF]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 8xms is ON HOLD  until Paper Publication
+==Crystal structure of Porcine Circovirus type II Rep ATPase domain==
+<StructureSection load='8xms' size='340' side='right'caption='[[8xms]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
-Authors:
+== Structural highlights ==
+<table><tr><td colspan='2'>[[8xms]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Porcine_circovirus_2 Porcine circovirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8XMS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8XMS FirstGlance]. <br>
-Description:
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
-[[Category: Unreleased Structures]]
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8xms FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8xms OCA], [https://pdbe.org/8xms PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8xms RCSB], [https://www.ebi.ac.uk/pdbsum/8xms PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8xms ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/REP_PCV2 REP_PCV2] Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep and/or Rep' binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the conserved sequence 5'-AGTATTAC-3' in the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, and nucleotidyl transferase.<ref>PMID:12954212</ref> <ref>PMID:25768890</ref>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Porcine circovirus 2]]
+[[Category: Guan SY]]
+[[Category: Song YF]]