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{{Seed}}
[[Image:2biw.png|left|200px]]


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==Crystal structure of apocarotenoid cleavage oxygenase from Synechocystis, native enzyme==
The line below this paragraph, containing "STRUCTURE_2biw", creates the "Structure Box" on the page.
<StructureSection load='2biw' size='340' side='right'caption='[[2biw]], [[Resolution|resolution]] 2.39&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2biw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp._PCC_6803 Synechocystis sp. PCC 6803]. The June 2005 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Carotenoid Oxygenase''  by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2005_6 10.2210/rcsb_pdb/mom_2005_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BIW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BIW FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.39&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3ON:(3R)-3-HYDROXY-8-APOCAROTENOL'>3ON</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
{{STRUCTURE_2biw|  PDB=2biw  |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2biw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2biw OCA], [https://pdbe.org/2biw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2biw RCSB], [https://www.ebi.ac.uk/pdbsum/2biw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2biw ProSAT]</span></td></tr>
 
</table>
===CRYSTAL STRUCTURE OF APOCAROTENOID CLEAVAGE OXYGENASE FROM SYNECHOCYSTIS, NATIVE ENZYME===
== Function ==
 
[https://www.uniprot.org/uniprot/ACOX_SYNY3 ACOX_SYNY3] Cleaves a number of carotenals and carotenols in the all-trans configuration at the 15-15' double bond producing retinal or retinol, respectively. Also shows activity toward lycopenals and the corresponding alcohols. Does not cleave beta-carotene or lycopene.
 
== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bi/2biw_consurf.spt"</scriptWhenChecked>
{{ABSTRACT_PUBMED_15821095}}
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==About this Structure==
  </jmolCheckbox>
2BIW is a 4 chains structure with sequences from [http://en.wikipedia.org/wiki/Synechocystis_sp. Synechocystis sp.]. The June 2005 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Carotenoid Oxygenase''  by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2005_6 10.2210/rcsb_pdb/mom_2005_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BIW OCA].  
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2biw ConSurf].
 
<div style="clear:both"></div>
==Reference==
__TOC__
<ref group="xtra">PMID:15821095</ref><references group="xtra"/>
</StructureSection>
[[Category: Carotenoid Oxygenase]]
[[Category: Carotenoid Oxygenase]]
[[Category: Large Structures]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Synechocystis sp.]]
[[Category: Synechocystis sp. PCC 6803]]
[[Category: Al-Babili, S.]]
[[Category: Al-Babili S]]
[[Category: Beyer, P.]]
[[Category: Beyer P]]
[[Category: Kloer, D P.]]
[[Category: Kloer DP]]
[[Category: Ruch, S.]]
[[Category: Ruch S]]
[[Category: Schulz, G E.]]
[[Category: Schulz GE]]
[[Category: Carotenoid cleavage]]
[[Category: Dioxygenase]]
[[Category: Non-heme iron]]
[[Category: Oxidoreductase]]
[[Category: Oxygenase]]
[[Category: Retinal formation]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jan 21 09:29:34 2010''

Latest revision as of 06:38, 1 May 2024

Crystal structure of apocarotenoid cleavage oxygenase from Synechocystis, native enzyme

2biw, resolution 2.39Å

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