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==Structure ensembles of duplex DNA containing a 4'-oxidized abasic site.==
==Structure ensembles of duplex DNA containing a 4'-oxidized abasic site.==
<StructureSection load='2hou' size='340' side='right'caption='[[2hou]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
<StructureSection load='2hou' size='340' side='right'caption='[[2hou]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2hou]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HOU OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2HOU FirstGlance]. <br>
<table><tr><td colspan='2'>[[2hou]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HOU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HOU FirstGlance]. <br>
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=X4A:[(2R,3S,5S)-2,3,5-TRIHYDROXYTETRAHYDROFURAN-2-YL]METHYL+DIHYDROGEN+PHOSPHATE'>X4A</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2hpx|2hpx]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=X4A:[(2R,3S,5S)-2,3,5-TRIHYDROXYTETRAHYDROFURAN-2-YL]METHYL+DIHYDROGEN+PHOSPHATE'>X4A</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2hou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hou OCA], [http://pdbe.org/2hou PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hou RCSB], [http://www.ebi.ac.uk/pdbsum/2hou PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2hou ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hou OCA], [https://pdbe.org/2hou PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hou RCSB], [https://www.ebi.ac.uk/pdbsum/2hou PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hou ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A 4'-oxidized abasic site (X) has been synthesized in a defined duplex DNA sequence, 5'-d(CCAAAGXACCGGG)-3'/3'-d(GGTTTCATGGCCC)-5' (1). Its structure has been determined by two-dimensional NMR methods, molecular modeling, and molecular dynamics simulations. 1 is globally B-form with the base (A) opposite X intrahelical and well-stacked. Only the alpha anomer of X is observed, and the abasic site deoxyribose is largely intrahelical. These results are compared with a normal abasic site (Y) in the same sequence context (2). Y is composed of a 60:40 mixture of alpha and beta anomers (2alpha and 2beta). In both 2alpha and 2beta, the base (A) opposite Y is intrahelical and well-stacked and the abasic site deoxyribose is predominantly extrahelical, consistent with the reported structures of the normal abasic site in a similar sequence context [Hoehn, S. T., Turner, C. J., and Stubbe, J. (2001) Nucleic Acids Res. 29, 3413-3423]. Molecular dynamics simulations reveal that the normal abasic site appears to be conformationally more flexible than the 4'-oxidized abasic site. The importance of the structure and flexibility of the abasic site in the recognition by the DNA repair enzyme Ape1 is discussed.
Nuclear magnetic resonance structural studies and molecular modeling of duplex DNA containing normal and 4'-oxidized abasic sites.,Chen J, Dupradeau FY, Case DA, Turner CJ, Stubbe J Biochemistry. 2007 Mar 20;46(11):3096-107. Epub 2007 Feb 27. PMID:17323932<ref>PMID:17323932</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2hou" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Case, D A]]
[[Category: Synthetic construct]]
[[Category: Chen, J]]
[[Category: Case DA]]
[[Category: Dupradeau, F Y]]
[[Category: Chen J]]
[[Category: Stubbe, J]]
[[Category: Dupradeau FY]]
[[Category: Turner, C J]]
[[Category: Stubbe J]]
[[Category: Abasic site]]
[[Category: Turner CJ]]
[[Category: Ber]]
[[Category: Bleomycin]]
[[Category: Dna]]
[[Category: Dna lesion]]
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