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==NMR structure of human Tra2beta1 RRM in complex with AAGAAC RNA==
==NMR structure of human Tra2beta1 RRM in complex with AAGAAC RNA==
<StructureSection load='2kxn' size='340' side='right' caption='[[2kxn]], [[NMR_Ensembles_of_Models | 12 NMR models]]' scene=''>
<StructureSection load='2kxn' size='340' side='right'caption='[[2kxn]]' scene=''>
== Structural highlights ==
== Structural highlights ==
[[2kxn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KXN OCA]. <br>
<table><tr><td colspan='2'>[[2kxn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KXN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KXN FirstGlance]. <br>
<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<b>Resources:</b> <span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kxn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kxn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kxn RCSB], [http://www.ebi.ac.uk/pdbsum/2kxn PDBsum]</span><br>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kxn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kxn OCA], [https://pdbe.org/2kxn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kxn RCSB], [https://www.ebi.ac.uk/pdbsum/2kxn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kxn ProSAT]</span></td></tr>
== Publication Abstract from PubMed ==
</table>
Tra2-beta1 is a unique splicing factor as its single RNA recognition motif (RRM) is located between two RS (arginine-serine) domains. To understand how this protein recognizes its RNA target, we solved the structure of Tra2-beta1 RRM in complex with RNA. The central 5'-AGAA-3' motif is specifically recognized by residues from the beta-sheet of the RRM and by residues from both extremities flanking the RRM. The structure suggests that RNA binding by Tra2-beta1 induces positioning of the two RS domains relative to one another. By testing the effect of Tra2-beta1 and RNA mutations on the splicing of SMN2 exon 7, we validated the importance of the RNA-protein contacts observed in the structure for the function of Tra2-beta1 and determined the functional sequence of Tra2-beta1 in SMN2 exon 7. Finally, we propose a model for the assembly of multiple RNA binding proteins on this exon.
== Function ==
 
[https://www.uniprot.org/uniprot/TRA2B_HUMAN TRA2B_HUMAN] Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing. Can either activate or suppress exon inclusion. Acts additively with RBMX to promote exon 7 inclusion of the survival motor neuron SMN2. Activates the splicing of MAPT/Tau exon 10. Alters pre-mRNA splicing patterns by antagonizing the effects of splicing regulators, like RBMX. Binds to the AG-rich SE2 domain in the SMN exon 7 RNA. Binds to pre-mRNA.<ref>PMID:9546399</ref> <ref>PMID:12165565</ref> <ref>PMID:12761049</ref> <ref>PMID:15009664</ref>  
Molecular basis of purine-rich RNA recognition by the human SR-like protein Tra2-beta1.,Clery A, Jayne S, Benderska N, Dominguez C, Stamm S, Allain FH Nat Struct Mol Biol. 2011 Apr;18(4):443-50. Epub 2011 Mar 13. PMID:21399644<ref>PMID:21399644</ref>
 
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Allain, F H.T.]]
[[Category: Large Structures]]
[[Category: Benderska, N.]]
[[Category: Allain FH-T]]
[[Category: Clery, A.]]
[[Category: Benderska N]]
[[Category: Dominguez, C.]]
[[Category: Clery A]]
[[Category: Jayne, S.]]
[[Category: Dominguez C]]
[[Category: Stamm, S.]]
[[Category: Jayne S]]
[[Category: Rna binding protein-rna complex]]
[[Category: Stamm S]]
[[Category: Rna protein complex]]
[[Category: Rrm]]
[[Category: Smn]]
[[Category: Splicing factor]]
[[Category: Sr protein]]
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