|
|
| (4 intermediate revisions by the same user not shown) |
| Line 1: |
Line 1: |
| | |
| ==Structure of a (3+1) G-quadruplex formed by hTERT promoter sequence== | | ==Structure of a (3+1) G-quadruplex formed by hTERT promoter sequence== |
| <StructureSection load='2kzd' size='340' side='right' caption='[[2kzd]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | | <StructureSection load='2kzd' size='340' side='right'caption='[[2kzd]]' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[2kzd]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KZD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KZD FirstGlance]. <br> | | <table><tr><td colspan='2'>[[2kzd]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KZD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KZD FirstGlance]. <br> |
| </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DI:2-DEOXYINOSINE-5-MONOPHOSPHATE'>DI</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2kze|2kze]], [[2a5p|2a5p]], [[2o3m|2o3m]]</td></tr>
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kzd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kzd OCA], [https://pdbe.org/2kzd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kzd RCSB], [https://www.ebi.ac.uk/pdbsum/2kzd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kzd ProSAT]</span></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kzd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kzd OCA], [http://pdbe.org/2kzd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kzd RCSB], [http://www.ebi.ac.uk/pdbsum/2kzd PDBsum]</span></td></tr> | |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| |
| == Publication Abstract from PubMed ==
| |
| The catalytic subunit of human telomerase, hTERT, actively elongates the 3' end of the telomere in most cancer cells. The hTERT promoter, which contains many guanine-rich stretches on the same DNA strand, exhibits an exceptional potential for G-quadruplex formation. Here we show that one particular G-rich sequence in this region coexists in two G-quadruplex conformations in potassium solution: a (3 + 1) and a parallel-stranded G-quadruplexes. We present the NMR solution structures of both conformations, each comprising several robust structural elements, among which include the (3 + 1) and all-parallel G-tetrad cores, single-residue double-chain-reversal loops, and a capping A.T base pair. A combination of NMR and CD techniques, complemented with sequence modifications and variations of experimental condition, allowed us to better understand the coexistence of the two G-quadruplex conformations in equilibrium and how different structural elements conspire to favor a particular form.
| |
|
| |
| Coexistence of two distinct G-quadruplex conformations in the hTERT promoter.,Lim KW, Lacroix L, Yue DJ, Lim JK, Lim JM, Phan AT J Am Chem Soc. 2010 Sep 8;132(35):12331-42. PMID:20704263<ref>PMID:20704263</ref>
| |
|
| |
| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| |
| </div>
| |
| <div class="pdbe-citations 2kzd" style="background-color:#fffaf0;"></div>
| |
| == References ==
| |
| <references/>
| |
| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Lacroix, L]] | | [[Category: Large Structures]] |
| [[Category: Lim, J K.C]] | | [[Category: Lacroix L]] |
| [[Category: Lim, J M.W]] | | [[Category: Lim JKC]] |
| [[Category: Lim, K W]] | | [[Category: Lim JMW]] |
| [[Category: Phan, A T]] | | [[Category: Lim KW]] |
| [[Category: Yue, D J.E]] | | [[Category: Phan AT]] |
| [[Category: Anticancer target]] | | [[Category: Yue DJE]] |
| [[Category: Dna]]
| |
| [[Category: G-quadruplex]]
| |
| [[Category: Htert promoter]]
| |
| [[Category: Telomerase inhibition]]
| |
