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| ==Solution structure of the rhodanese domain of YgaP from E. coli== | | ==Solution structure of the rhodanese domain of YgaP from E. coli== |
| <StructureSection load='2mrm' size='340' side='right' caption='[[2mrm]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | | <StructureSection load='2mrm' size='340' side='right'caption='[[2mrm]]' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[2mrm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MRM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MRM FirstGlance]. <br> | | <table><tr><td colspan='2'>[[2mrm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MRM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MRM FirstGlance]. <br> |
| </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BU34_15760, CF57_07085, CF61_07785 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mrm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mrm OCA], [http://pdbe.org/2mrm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mrm RCSB], [http://www.ebi.ac.uk/pdbsum/2mrm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2mrm ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mrm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mrm OCA], [https://pdbe.org/2mrm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mrm RCSB], [https://www.ebi.ac.uk/pdbsum/2mrm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mrm ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| Rhodanese domains are abundant structural modules that catalyze the transfer of a sulfur atom from thiolsulfates to cyanide via formation of a covalent persulfide intermediate that is bound to an essential conserved cysteine residue. In this study, the three-dimensional structure of the rhodanese domain of YgaP from Escherichia coli was determined using solution NMR. A typical rhodanese domain fold was observed, as expected from the high homology with the catalytic domain of other sulfur transferases. The initial sulfur-transfer step and formation of the rhodanese persulfide intermediate were monitored by addition of sodium thiosulfate using two-dimensional (1)H-(15)N correlation spectroscopy. Discrete sharp signals were observed upon substrate addition, indicting fast exchange between sulfur-free and persulfide-intermediate forms. Residues exhibiting pronounced chemical shift changes were mapped to the structure, and included both substrate binding and surrounding residues.
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| Fast conformational exchange between the sulfur-free and persulfide-bound rhodanese domain of E. coli YgaP.,Wang W, Zhou P, He Y, Yu L, Xiong Y, Tian C, Wu F Biochem Biophys Res Commun. 2014 Sep 26;452(3):817-21. doi:, 10.1016/j.bbrc.2014.09.002. Epub 2014 Sep 7. PMID:25204500<ref>PMID:25204500</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 2mrm" style="background-color:#fffaf0;"></div>
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| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Bacillus coli migula 1895]] | | [[Category: Escherichia coli]] |
| [[Category: Tian, C]] | | [[Category: Large Structures]] |
| [[Category: Wang, W]] | | [[Category: Tian C]] |
| [[Category: Wu, F]] | | [[Category: Wang W]] |
| [[Category: Zhou, P]] | | [[Category: Wu F]] |
| [[Category: E. coli]]
| | [[Category: Zhou P]] |
| [[Category: Integral membrane protein]]
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| [[Category: Membrane protein]]
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| [[Category: Rhodanese domain]]
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| [[Category: Ygap]]
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