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[[Image:2ckz.gif|left|200px]]
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{{STRUCTURE_2ckz|  PDB=2ckz  |  SCENE=  }}
'''X-RAY STRUCTURE OF RNA POLYMERASE III SUBCOMPLEX C17-C25.'''


==X-ray structure of RNA polymerase III subcomplex C17-C25.==
<StructureSection load='2ckz' size='340' side='right'caption='[[2ckz]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2ckz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CKZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CKZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ckz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ckz OCA], [https://pdbe.org/2ckz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ckz RCSB], [https://www.ebi.ac.uk/pdbsum/2ckz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ckz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RPC9_YEAST RPC9_YEAST] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. The RPC25/RPC8-RPC17/RPC9 subcomplex may bind Pol III transcripts emerging from the adjacent exit pore during elongation.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ck/2ckz_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ckz ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We obtained an 11 subunit model of RNA polymerase (Pol) III by combining a homology model of the nine subunit core enzyme with a new X-ray structure of the subcomplex C17/25. Compared to Pol II, Pol III shows a conserved active center for RNA synthesis but a structurally different upstream face for specific initiation complex assembly during promoter selection. The Pol III upstream face includes a HRDC domain in subunit C17 that is translated by 35 A and rotated by 150 degrees compared to its Pol II counterpart. The HRDC domain is essential in vivo, folds independently in vitro, and, unlike other HRDC domains, shows no indication of nucleic acid binding. Thus, the HRDC domain is a functional module that could account for the role of C17 in Pol III promoter-specific initiation. During elongation, C17/25 may bind Pol III transcripts emerging from the adjacent exit pore, because the subcomplex binds to tRNA in vitro.


==Overview==
Structural biology of RNA polymerase III: subcomplex C17/25 X-ray structure and 11 subunit enzyme model.,Jasiak AJ, Armache KJ, Martens B, Jansen RP, Cramer P Mol Cell. 2006 Jul 7;23(1):71-81. PMID:16818233<ref>PMID:16818233</ref>
We obtained an 11 subunit model of RNA polymerase (Pol) III by combining a homology model of the nine subunit core enzyme with a new X-ray structure of the subcomplex C17/25. Compared to Pol II, Pol III shows a conserved active center for RNA synthesis but a structurally different upstream face for specific initiation complex assembly during promoter selection. The Pol III upstream face includes a HRDC domain in subunit C17 that is translated by 35 A and rotated by 150 degrees compared to its Pol II counterpart. The HRDC domain is essential in vivo, folds independently in vitro, and, unlike other HRDC domains, shows no indication of nucleic acid binding. Thus, the HRDC domain is a functional module that could account for the role of C17 in Pol III promoter-specific initiation. During elongation, C17/25 may bind Pol III transcripts emerging from the adjacent exit pore, because the subcomplex binds to tRNA in vitro.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
2CKZ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CKZ OCA].
</div>
<div class="pdbe-citations 2ckz" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Structural biology of RNA polymerase III: subcomplex C17/25 X-ray structure and 11 subunit enzyme model., Jasiak AJ, Armache KJ, Martens B, Jansen RP, Cramer P, Mol Cell. 2006 Jul 7;23(1):71-81. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16818233 16818233]
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
[[Category: DNA-directed RNA polymerase]]
== References ==
[[Category: Protein complex]]
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Armache, K J.]]
[[Category: Armache K-J]]
[[Category: Cramer, P.]]
[[Category: Cramer P]]
[[Category: Jansen, R P.]]
[[Category: Jansen R-P]]
[[Category: Jasiak, A J.]]
[[Category: Jasiak AJ]]
[[Category: Martens, B.]]
[[Category: Martens B]]
[[Category: Class iii gene transcription]]
[[Category: Dna-directed rna polymerase]]
[[Category: Eukaryotic nucleic acid polymerase]]
[[Category: Hypothetical protein]]
[[Category: Multiprotein complex]]
[[Category: Nuclear protein]]
[[Category: Nucleotidyltransferase]]
[[Category: Transcription]]
[[Category: Transferase]]
[[Category: Trna synthesis]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 22:23:30 2008''
Retrieved from "https://proteopedia.org/2ckz"