3zu8: Difference between revisions

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[[Image:3zu8.png|left|200px]]


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==STRUCTURE OF CBM3B OF MAJOR SCAFFOLDIN SUBUNIT SCAA FROM ACETIVIBRIO CELLULOLYTICUS DETERMINED ON THE NIKEL ABSORPTION EDGE==
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<StructureSection load='3zu8' size='340' side='right'caption='[[3zu8]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3zu8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Acetivibrio_cellulolyticus Acetivibrio cellulolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZU8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZU8 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.801&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
{{STRUCTURE_3zu8|  PDB=3zu8  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zu8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zu8 OCA], [https://pdbe.org/3zu8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zu8 RCSB], [https://www.ebi.ac.uk/pdbsum/3zu8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zu8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9RPL0_9FIRM Q9RPL0_9FIRM]
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== Publication Abstract from PubMed ==
The carbohydrate-binding module (CBM) of the major scaffoldin subunit ScaA of the cellulosome of Acetivibrio cellulolyticus is classified as a family 3b CBM and binds strongly to cellulose. The CBM3b was overexpressed, purified and crystallized, and its three-dimensional structure was determined. The structure contained a nickel-binding site located at the N-terminal region in addition to a 'classical' CBM3b calcium-binding site. The structure was also determined independently by the SAD method using data collected at the Ni-absorption wavelength of 1.48395 A and even at a wavelength of 0.97625 A in a favourable case. The new scaffoldin-borne CBM3 structure reported here provides clear evidence for the proposition that a family 3b CBM may be accommodated in scaffoldin subunits and functions as the major substrate-binding entity of the cellulosome assembly.


===STRUCTURE OF CBM3B OF MAJOR SCAFFOLDIN SUBUNIT SCAA FROM ACETIVIBRIO CELLULOLYTICUS DETERMINED ON THE NIKEL ABSORPTION EDGE===
Structure of CBM3b of the major cellulosomal scaffoldin subunit ScaA from Acetivibrio cellulolyticus.,Yaniv O, Halfon Y, Shimon LJ, Bayer EA, Lamed R, Frolow F Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Jan 1;68(Pt 1):8-13. Epub , 2011 Dec 24. PMID:22232162<ref>PMID:22232162</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
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<references/>
{{ABSTRACT_PUBMED_22232162}}
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</StructureSection>
==About this Structure==
[[3zu8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Acetivibrio_cellulolyticus Acetivibrio cellulolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZU8 OCA].
 
==Reference==
<ref group="xtra">PMID:022232162</ref><references group="xtra"/>
[[Category: Acetivibrio cellulolyticus]]
[[Category: Acetivibrio cellulolyticus]]
[[Category: Frolow, F.]]
[[Category: Large Structures]]
[[Category: Halfon, Y.]]
[[Category: Frolow F]]
[[Category: Lamed, R.]]
[[Category: Halfon Y]]
[[Category: Yaniv, O.]]
[[Category: Lamed R]]
[[Category: Cellulosome]]
[[Category: Yaniv O]]
[[Category: Sugar binding protein]]