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New page: '''Unreleased structure''' The entry 4umm is ON HOLD Authors: Maletta, M., Orlov, I., Moras, D., Billas, I.M.L., Klaholz, B.P. Description: The Cryo-EM structure of the palindromic DNA...
 
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'''Unreleased structure'''


The entry 4umm is ON HOLD
==The Cryo-EM structure of the palindromic DNA-bound USP-EcR nuclear receptor reveals an asymmetric organization with allosteric domain positioning==
<SX load='4umm' size='340' side='right' viewer='molstar' caption='[[4umm]], [[Resolution|resolution]] 11.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4umm]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Heliothis_virescens Heliothis virescens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UMM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UMM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 11.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPH:L-ALPHA-PHOSPHATIDYL-BETA-OLEOYL-GAMMA-PALMITOYL-PHOSPHATIDYLETHANOLAMINE'>EPH</scene>, <scene name='pdbligand=P1A:2,3,14,20,22-PENTAHYDROXYCHOLEST-7-EN-6-ONE'>P1A</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4umm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4umm OCA], [https://pdbe.org/4umm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4umm RCSB], [https://www.ebi.ac.uk/pdbsum/4umm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4umm ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ECR_HELVI ECR_HELVI] Receptor for ecdysone. Binds to ecdysone response elements (ECRES) (By similarity).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Nuclear receptors (NRs) regulate gene expression through DNA- and ligand-binding and thus represent crucial therapeutic targets. The ultraspiracle protein/ecdysone receptor (USP/EcR) complex binds to half-sites with a one base pair spaced inverted repeat (IR1), a palindromic DNA response element (RE) reminiscent of IRs observed for vertebrate steroid hormone receptors. Here we present the cryo electron microscopy structure of the USP/EcR complex bound to an IR1 RE which provides the first description of a full IR-bound NR complex. The structure reveals that even though the DNA is almost symmetric, the complex adopts a highly asymmetric architecture in which the ligand-binding domains (LBDs) are positioned 5' off-centred. Additional interactions of the USP LBD with the 5'-flanking sequence trigger transcription activity as monitored by transfection assays. The comparison with DR-bound NR complexes suggests that DNA is the major allosteric driver in inversely positioning the LBDs, which serve as the main binding-site for transcriptional regulators.


Authors: Maletta, M., Orlov, I., Moras, D., Billas, I.M.L., Klaholz, B.P.
The palindromic DNA-bound USP/EcR nuclear receptor adopts an asymmetric organization with allosteric domain positioning.,Maletta M, Orlov I, Roblin P, Beck Y, Moras D, Billas IM, Klaholz BP Nat Commun. 2014 Jun 19;5:4139. doi: 10.1038/ncomms5139. PMID:24942373<ref>PMID:24942373</ref>


Description: The Cryo-EM structure of the palindromic DNA-bound USP-EcR nuclear receptor reveals an asymmetric organization with allosteric domain positioning
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4umm" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</SX>
[[Category: Heliothis virescens]]
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Billas IML]]
[[Category: Klaholz BP]]
[[Category: Maletta M]]
[[Category: Moras D]]
[[Category: Orlov I]]
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