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{{STRUCTURE_2m9m|  PDB=2m9m  |  SCENE=  }}
===Solution Structure of ERCC4 domain of human FAAP24===
{{ABSTRACT_PUBMED_23999858}}


==Function==
==Solution Structure of ERCC4 domain of human FAAP24==
[[http://www.uniprot.org/uniprot/FAP24_HUMAN FAP24_HUMAN]] Plays a role in DNA repair through recruitment of the FA core complex to damaged DNA. Regulates FANCD2 monoubiquitination upon DNA damage. Induces chromosomal instability as well as hypersensitivity to DNA cross-linking agents, when repressed. Targets FANCM/FAAP24 complex to the DNA, preferentially to single strand DNA.  
<StructureSection load='2m9m' size='340' side='right'caption='[[2m9m]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2m9m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M9M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M9M FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m9m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m9m OCA], [https://pdbe.org/2m9m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m9m RCSB], [https://www.ebi.ac.uk/pdbsum/2m9m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m9m ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/FAP24_HUMAN FAP24_HUMAN] Plays a role in DNA repair through recruitment of the FA core complex to damaged DNA. Regulates FANCD2 monoubiquitination upon DNA damage. Induces chromosomal instability as well as hypersensitivity to DNA cross-linking agents, when repressed. Targets FANCM/FAAP24 complex to the DNA, preferentially to single strand DNA.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The FANCM/FAAP24 heterodimer has distinct functions in protecting cells from complex DNA lesions such as interstrand crosslinks. These functions rely on the biochemical activity of FANCM/FAAP24 to recognize and bind to damaged DNA or stalled replication forks. However, the DNA-binding activity of this complex was not clearly defined. We investigated how FAAP24 contributes to the DNA-interacting functions of the FANCM/FAAP24 complex by acquiring the N-terminal and C-terminal solution structures of human FAAP24. Modeling of the FAAP24 structure indicates that FAAP24 may possess a high affinity toward single-stranded DNA (ssDNA). Testing of various FAAP24 mutations in vitro and in vivo validated this prediction derived from structural analyses. We found that the DNA-binding and FANCM-interacting functions of FAAP24, although both require the C-terminal (HhH)2 domain, can be distinguished by segregation-of-function mutations. These results demonstrate dual roles of FAAP24 in DNA damage response against crosslinking lesions, one through the formation of FANCM/FAAP24 heterodimer and the other via its ssDNA-binding activity required in optimized checkpoint activation.Cell Research advance online publication 3 September 2013; doi:10.1038/cr.2013.124.


==About this Structure==
Structure analysis of FAAP24 reveals single-stranded DNA-binding activity and domain functions in DNA damage response.,Wang Y, Han X, Wu F, Leung JW, Lowery MG, Do H, Chen J, Shi C, Tian C, Li L, Gong W Cell Res. 2013 Sep 3. doi: 10.1038/cr.2013.124. PMID:23999858<ref>PMID:23999858</ref>
[[2m9m]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M9M OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
<ref group="xtra">PMID:023999858</ref><references group="xtra"/><references/>
</div>
<div class="pdbe-citations 2m9m" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Gong, W.]]
[[Category: Large Structures]]
[[Category: Han, X.]]
[[Category: Gong W]]
[[Category: Shi, C.]]
[[Category: Han X]]
[[Category: Tian, C.]]
[[Category: Shi C]]
[[Category: Wu, F.]]
[[Category: Tian C]]
[[Category: Dna binding protein]]
[[Category: Wu F]]
[[Category: Ercc4 domain]]
[[Category: Faap24]]
[[Category: Fanconi anemia]]
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