2bw3: Difference between revisions
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==Three-dimensional structure of the Hermes DNA transposase== | |||
| | <StructureSection load='2bw3' size='340' side='right'caption='[[2bw3]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2bw3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Musca_domestica Musca domestica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BW3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BW3 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bw3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bw3 OCA], [https://pdbe.org/2bw3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bw3 RCSB], [https://www.ebi.ac.uk/pdbsum/2bw3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bw3 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q25438_MUSDO Q25438_MUSDO] | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bw/2bw3_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bw3 ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Mobile elements and their inactive remnants account for large proportions of most eukaryotic genomes, where they have had central roles in genome evolution. Over 50 years ago, McClintock reported a form of stress-induced genome instability in maize in which discrete DNA segments move between chromosomal locations. Our current mechanistic understanding of enzymes catalyzing transposition is largely limited to prokaryotic transposases. The Hermes transposon from the housefly is part of the eukaryotic hAT superfamily that includes hobo from Drosophila, McClintock's maize Activator and Tam3 from snapdragon. We report here the three-dimensional structure of a functionally active form of the transposase from Hermes at 2.1-A resolution. The Hermes protein has some structural features of prokaryotic transposases, including a domain with a retroviral integrase fold. However, this domain is disrupted by the insertion of an additional domain. Finally, transposition is observed only when Hermes assembles into a hexamer. | |||
Molecular architecture of a eukaryotic DNA transposase.,Hickman AB, Perez ZN, Zhou L, Musingarimi P, Ghirlando R, Hinshaw JE, Craig NL, Dyda F Nat Struct Mol Biol. 2005 Aug;12(8):715-21. Epub 2005 Jul 24. PMID:16041385<ref>PMID:16041385</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2bw3" style="background-color:#fffaf0;"></div> | |||
== | ==See Also== | ||
*[[Transposase 3D structures|Transposase 3D structures]] | |||
== References == | |||
== | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Musca domestica]] | [[Category: Musca domestica]] | ||
[[Category: Craig NL]] | |||
[[Category: Craig | [[Category: Dyda F]] | ||
[[Category: Dyda | [[Category: Ghirlando R]] | ||
[[Category: Ghirlando | [[Category: Hickman AB]] | ||
[[Category: Hickman | [[Category: Hinshaw JE]] | ||
[[Category: Hinshaw | [[Category: Musingarimi P]] | ||
[[Category: Musingarimi | [[Category: Perez ZN]] | ||
[[Category: Perez | [[Category: Zhou L]] | ||
[[Category: Zhou | |||
Latest revision as of 11:24, 22 May 2024
Three-dimensional structure of the Hermes DNA transposase
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