2fei: Difference between revisions

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{{Seed}}
[[Image:2fei.png|left|200px]]


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==Solution structure of the second SH3 domain of Human CMS protein==
The line below this paragraph, containing "STRUCTURE_2fei", creates the "Structure Box" on the page.
<StructureSection load='2fei' size='340' side='right'caption='[[2fei]]' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2fei]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FEI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FEI FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-->
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fei FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fei OCA], [https://pdbe.org/2fei PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fei RCSB], [https://www.ebi.ac.uk/pdbsum/2fei PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fei ProSAT]</span></td></tr>
{{STRUCTURE_2fei|  PDB=2fei  |  SCENE=  }}
</table>
== Disease ==
[https://www.uniprot.org/uniprot/CD2AP_HUMAN CD2AP_HUMAN] Defects in CD2AP are the cause of susceptibility to focal segmental glomerulosclerosis type 3 (FSGS3) [MIM:[https://omim.org/entry/607832 607832]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.<ref>PMID:12764198</ref>
== Function ==
[https://www.uniprot.org/uniprot/CD2AP_HUMAN CD2AP_HUMAN] Seems to act as an adapter protein between membrane proteins and the actin cytoskeleton. May play a role in receptor clustering and cytoskeletal polarity in the junction between T-cell and antigen-presenting cell. May anchor the podocyte slit diaphragm to the actin cytoskeleton in renal glomerolus. Also required for cytokinesis.<ref>PMID:15800069</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fe/2fei_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fei ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
CMS, cas ligand with multiple Src homology 3 (SH3) domains, belongs to a family of ubiquitously expressed adaptor proteins. Among the CMS binding proteins, c-Cbl has been mostly extensively studied. It was reported that the motif PKPFPR (residues 824-829) of c-Cbl can bind to the N-terminus SH3 domains of CMS. Here we report the solution structure of the second SH3 domain of CMS (CMS_SH3_B), furthermore, we have identified that a peptide from residues 701 to 714 of c-Cbl (Cbl-p), i.e. MTPSSRPLRPLDTS, can specially bind to CMS_SH3_B using NMR chemical shift perturbation, suggesting that the peptide is a new potential CMS binding site. Among the peptide, TPSSRPLR is the core binding motif and Arg709 plays a key role in the interaction. Cbl-p binding interface on CMS_SH3_B along a hydrophobic channel is composed of RT loop, n-Src loop and beta4 strand and divided into three pockets. This work indicates the solution structure of CMS_SH3_B bears the canonical beta-beta-beta-beta-alpha-beta fold and a new binding site in c-Cbl involved in its interaction with CMS, which probably contributes to the clustering of CMS. All the information provided here should be beneficial for the future functional study of CMS.


===Solution structure of the second SH3 domain of Human CMS protein===
Solution structure of the second SH3 domain of human CMS and a newly identified binding site at the C-terminus of c-Cbl.,Yao B, Zhang J, Dai H, Sun J, Jiao Y, Tang Y, Wu J, Shi Y Biochim Biophys Acta. 2007 Jan;1774(1):35-43. Epub 2006 Oct 27. PMID:17188587<ref>PMID:17188587</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2fei" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_17188587}}, adds the Publication Abstract to the page
*[[CD2-associated protein 3D structures|CD2-associated protein 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 17188587 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_17188587}}
__TOC__
 
</StructureSection>
==Disease==
Known disease associated with this structure: Glomerulosclerosis, focal segmental, 3 OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=604241 604241]]
 
==About this Structure==
2FEI is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FEI OCA].
 
==Reference==
<ref group="xtra">PMID:17188587</ref><references group="xtra"/>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Dai, H.]]
[[Category: Large Structures]]
[[Category: Jiao, Y.]]
[[Category: Dai H]]
[[Category: Shi, Y.]]
[[Category: Jiao Y]]
[[Category: Wu, J.]]
[[Category: Shi Y]]
[[Category: Yao, B.]]
[[Category: Wu J]]
[[Category: Cms sh3 domain]]
[[Category: Yao B]]
 
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Retrieved from "https://proteopedia.org/2fei"