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New page: left|200px<br /><applet load="1cpc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cpc, resolution 1.66Å" /> '''ISOLATION, CRYSTALLI...
 
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[[Image:1cpc.gif|left|200px]]<br /><applet load="1cpc" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1cpc, resolution 1.66&Aring;" />
'''ISOLATION, CRYSTALLIZATION, CRYSTAL STRUCTURE ANALYSIS AND REFINEMENT OF CONSTITUTIVE C-PHYCOCYANIN FROM THE CHROMATICALLY ADAPTING CYANOBACTERIUM FREMYELLA DIPLOSIPHON AT 1.66 ANGSTROMS RESOLUTION'''<br />


==Overview==
==ISOLATION, CRYSTALLIZATION, CRYSTAL STRUCTURE ANALYSIS AND REFINEMENT OF CONSTITUTIVE C-PHYCOCYANIN FROM THE CHROMATICALLY ADAPTING CYANOBACTERIUM FREMYELLA DIPLOSIPHON AT 1.66 ANGSTROMS RESOLUTION==
Constitutive phycocyanin from cyanobacterium Fremyella diplosiphon, (Calothrix sp. PCC 7601) grown in green light, has been isolated and, crystallized. The crystals belong to the space group R3 with cell, constants a = b = 180.26 A, c = 61.24 A, alpha = beta = 90 degrees, gamma, = 120 degrees. The crystal structure has been determined by Patterson, search techniques using the molecular model of C-phycocyanin from the, cyanobacterium Agmenellum quadruplicatum. The asymmetric unit of the, crystal cell consists of two (alpha beta)-monomers related by a local, dyad. Three asymmetric units are arranged around a crystallographic triad, and form an (alpha beta)6-hexamer, the functional unit in the native, antenna rod. The initial structure has been refined in a cyclic manner by, energy-restrained crystallographic refinement and modelling until the, conventional crystallographic R-factor converged at 18.1% with data to a, resolution of 1.66 A. The molecular structure resembles closely the, C-phycocyanins of Mastigocladus laminosus and A. quadruplicatum. The, conformation and configuration of the alpha-84 and beta-84 chromophores is, very similar to the corresponding chromophores in the trimeric, C-phycocyanin of M. laminosus, whereas the beta-155 chromophore differs in, configuration with C(4)-Z, C(10)-Z and C(15)-Z compared to C(4)-Z, C(10)-Z, C(15)-Z,E. The stereochemistry of the beta-155 chiral centres is, C(2)-RC(3)-R and C(31)-S, respectively, whereas alpha-84 and beta-84 have, C(2)-RC(3)-R and C(31)-R. The amino acid sequences of constitutive and, inducible phycocyanin differ mainly in residues located on the surface of, the beta-subunits that mediate the inter-hexameric contacts.
<StructureSection load='1cpc' size='340' side='right'caption='[[1cpc]], [[Resolution|resolution]] 1.66&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1cpc]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Microchaete_diplosiphon Microchaete diplosiphon]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CPC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CPC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.66&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CYC:PHYCOCYANOBILIN'>CYC</scene>, <scene name='pdbligand=MEN:N-METHYL+ASPARAGINE'>MEN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cpc OCA], [https://pdbe.org/1cpc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cpc RCSB], [https://www.ebi.ac.uk/pdbsum/1cpc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cpc ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PHCA1_MICDP PHCA1_MICDP] Light-harvesting photosynthetic bile pigment-protein from the phycobiliprotein complex (phycobilisome, PBS). Phycocyanin is the major phycobiliprotein in the PBS rod.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cp/1cpc_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cpc ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Constitutive phycocyanin from cyanobacterium Fremyella diplosiphon (Calothrix sp. PCC 7601) grown in green light, has been isolated and crystallized. The crystals belong to the space group R3 with cell constants a = b = 180.26 A, c = 61.24 A, alpha = beta = 90 degrees, gamma = 120 degrees. The crystal structure has been determined by Patterson search techniques using the molecular model of C-phycocyanin from the cyanobacterium Agmenellum quadruplicatum. The asymmetric unit of the crystal cell consists of two (alpha beta)-monomers related by a local dyad. Three asymmetric units are arranged around a crystallographic triad and form an (alpha beta)6-hexamer, the functional unit in the native antenna rod. The initial structure has been refined in a cyclic manner by energy-restrained crystallographic refinement and modelling until the conventional crystallographic R-factor converged at 18.1% with data to a resolution of 1.66 A. The molecular structure resembles closely the C-phycocyanins of Mastigocladus laminosus and A. quadruplicatum. The conformation and configuration of the alpha-84 and beta-84 chromophores is very similar to the corresponding chromophores in the trimeric C-phycocyanin of M. laminosus, whereas the beta-155 chromophore differs in configuration with C(4)-Z, C(10)-Z and C(15)-Z compared to C(4)-Z, C(10)-Z, C(15)-Z,E. The stereochemistry of the beta-155 chiral centres is C(2)-RC(3)-R and C(31)-S, respectively, whereas alpha-84 and beta-84 have C(2)-RC(3)-R and C(31)-R. The amino acid sequences of constitutive and inducible phycocyanin differ mainly in residues located on the surface of the beta-subunits that mediate the inter-hexameric contacts.


==About this Structure==
Isolation, crystallization, crystal structure analysis and refinement of constitutive C-phycocyanin from the chromatically adapting cyanobacterium Fremyella diplosiphon at 1.66 A resolution.,Duerring M, Schmidt GB, Huber R J Mol Biol. 1991 Feb 5;217(3):577-92. PMID:1899708<ref>PMID:1899708</ref>
1CPC is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Fremyella_diplosiphon Fremyella diplosiphon] with CYC and CH2 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CPC OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Isolation, crystallization, crystal structure analysis and refinement of constitutive C-phycocyanin from the chromatically adapting cyanobacterium Fremyella diplosiphon at 1.66 A resolution., Duerring M, Schmidt GB, Huber R, J Mol Biol. 1991 Feb 5;217(3):577-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1899708 1899708]
</div>
[[Category: Fremyella diplosiphon]]
<div class="pdbe-citations 1cpc" style="background-color:#fffaf0;"></div>
[[Category: Protein complex]]
[[Category: Duerring, M.]]
[[Category: Huber, R.]]
[[Category: Schmidt, G.B.]]
[[Category: CH2]]
[[Category: CYC]]
[[Category: light harvesting protein]]


''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:38:42 2007''
==See Also==
*[[Phycocyanin|Phycocyanin]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Microchaete diplosiphon]]
[[Category: Duerring M]]
[[Category: Huber R]]
[[Category: Schmidt GB]]
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