2ghp: Difference between revisions

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{{Seed}}
[[Image:2ghp.png|left|200px]]


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==Crystal structure of the N-terminal 3 RNA binding domains of the yeast splicing factor Prp24==
The line below this paragraph, containing "STRUCTURE_2ghp", creates the "Structure Box" on the page.
<StructureSection load='2ghp' size='340' side='right'caption='[[2ghp]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2ghp]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GHP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GHP FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
{{STRUCTURE_2ghp|  PDB=2ghp  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ghp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ghp OCA], [https://pdbe.org/2ghp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ghp RCSB], [https://www.ebi.ac.uk/pdbsum/2ghp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ghp ProSAT], [https://www.topsan.org/Proteins/CESG/2ghp TOPSAN]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PRP24_YEAST PRP24_YEAST] Binds preferentially to the U4/U6 hybrid snRNAs. Can stimulate the annealing of U4 and U6. Could participate in both the formation and disassembly of the U4/U6 hybrid during splicing.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gh/2ghp_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ghp ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The essential Saccharomyces cerevisiae pre-messenger RNA splicing protein 24 (Prp24) has four RNA recognition motifs (RRMs) and facilitates U6 RNA base-pairing with U4 RNA during spliceosome assembly. Prp24 is a component of the free U6 small nuclear ribonucleoprotein particle (snRNP) but not the U4/U6 bi-snRNP, and so is thought to be displaced from U6 by U4/U6 base-pairing. The interaction partners of each of the four RRMs of Prp24 and how these interactions direct U4/U6 pairing are not known. Here we report the crystal structure of the first three RRMs and the solution structure of the first two RRMs of Prp24. Strikingly, RRM 2 forms extensive inter-domain contacts with RRMs 1 and 3. These contacts occupy much of the canonical RNA-binding faces (beta-sheets) of RRMs 1 and 2, but leave the beta-sheet of RRM 3 exposed. Previously identified substitutions in Prp24 that suppress mutations in U4 and U6 spliceosomal RNAs cluster primarily in the beta-sheet of RRM 3, but also in a conserved loop of RRM 2. RNA binding assays and chemical shift mapping indicate that a large basic patch evident on the surface of RRMs 1 and 2 is part of a high affinity U6 RNA binding site. Our results suggest that Prp24 binds free U6 RNA primarily with RRMs 1 and 2, which may remodel the U6 secondary structure. The beta-sheet of RRM 3 then influences U4/U6 pairing through interaction with an unidentified ligand.


===Crystal structure of the N-terminal 3 RNA binding domains of the yeast splicing factor Prp24===
Structure and interactions of the first three RNA recognition motifs of splicing factor prp24.,Bae E, Reiter NJ, Bingman CA, Kwan SS, Lee D, Phillips GN Jr, Butcher SE, Brow DA J Mol Biol. 2007 Apr 13;367(5):1447-58. Epub 2007 Feb 7. PMID:17320109<ref>PMID:17320109</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2ghp" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_17320109}}, adds the Publication Abstract to the page
*[[Pre-mRNA splicing factors 3D structures|Pre-mRNA splicing factors 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 17320109 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_17320109}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
2GHP is a 8 chains structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GHP OCA].
 
==Reference==
<ref group="xtra">PMID:17320109</ref><references group="xtra"/>
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Bae, E.]]
[[Category: Bae E]]
[[Category: Bingman, C A.]]
[[Category: Bingman CA]]
[[Category: Bitto, E.]]
[[Category: Bitto E]]
[[Category: CESG, Center for Eukaryotic Structural Genomics.]]
[[Category: Phillips Jr GN]]
[[Category: Jr., G N.Phillips.]]
[[Category: Wesenberg GE]]
[[Category: Wesenberg, G E.]]
[[Category: Center for eukaryotic structural genomic]]
[[Category: Cesg]]
[[Category: Protein structure initiative]]
[[Category: Psi]]
[[Category: Rna binding domain]]
[[Category: Rna binding protein]]
[[Category: Rna chaperone]]
[[Category: Rna recognition motif]]
[[Category: Snrnp]]
[[Category: Spliceosome]]
[[Category: Splicing factor]]
[[Category: Structural genomic]]
 
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Retrieved from "https://proteopedia.org/2ghp"