2i28: Difference between revisions

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[[Image:2i28.png|left|200px]]


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==Solution Structure of alpha-Conotoxin BuIA==
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<StructureSection load='2i28' size='340' side='right'caption='[[2i28]]' scene=''>
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== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2i28]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Conus_bullatus Conus bullatus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I28 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I28 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
{{STRUCTURE_2i28|  PDB=2i28  |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i28 OCA], [https://pdbe.org/2i28 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i28 RCSB], [https://www.ebi.ac.uk/pdbsum/2i28 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i28 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CA1A_CONBU CA1A_CONBU] Alpha-conotoxins bind to the nicotinic acetylcholine receptors (nAChR) and inhibit them. This peptide potently blocks numerous mammalian nAChR subtypes (alpha-6 or -3/beta-2 or -3 > alpha-6 or-3/beta-4 > alpha-3/beta-2 > alpha-3/beta-4 > alpha-4/beta-4 = alpha-2/beta-4 > alpha-7 > alpha-2/beta-2 >> alpha-4/beta-2). Recovery from toxin block is markedly slower for beta-4 versus beta-2 subunit-containing nAChRs. Thus, it represents a novel probe for distinguishing between beta-2 and beta-4-containing nAChRs.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We have determined a high-resolution three-dimensional structure of alpha-conotoxin BuIA, a 13-residue peptide toxin isolated from Conus bullatus. Despite its unusual 4/4 disulfide bond layout alpha-conotoxin BuIA exhibits strong antagonistic activity at alpha6/alpha3beta2beta3, alpha3beta2, and alpha3beta4 nAChR subtypes like some alpha4/7 conotoxins. alpha-Conotoxin BuIA lacks the C-terminal beta-turn present within the second disulfide loop of alpha4/7 conotoxins, having only a "pseudo omega-shaped" molecular topology. Nevertheless, it contains a functionally critical two-turn helix motif, a feature ubiquitously found in alpha4/7 conotoxins. Such an aspect seems mainly responsible for similarities in the receptor recognition profile of alpha-conotoxin BuIA to alpha4/7 conotoxins. Structural comparison of alpha-conotoxin BuIA with alpha4/7 conotoxins and alpha4/3 conotoxin ImI suggests that presence of the second helical turn portion of the two-turn helix motif in alpha4/7 and alpha4/4 conotoxins may be important for binding to the alpha3 and/or alpha6 subunit of nAChR.


===Solution Structure of alpha-Conotoxin BuIA===
NMR structure determination of alpha-conotoxin BuIA, a novel neuronal nicotinic acetylcholine receptor antagonist with an unusual 4/4 disulfide scaffold.,Chi SW, Kim DH, Olivera BM, McIntosh JM, Han KH Biochem Biophys Res Commun. 2006 Nov 3;349(4):1228-34. Epub 2006 Sep 7. PMID:16979596<ref>PMID:16979596</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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(as it appears on PubMed at http://www.pubmed.gov), where 16979596 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_16979596}}
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</StructureSection>
==About this Structure==
[[Category: Conus bullatus]]
2I28 is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I28 OCA].
[[Category: Large Structures]]
 
[[Category: Chi S-W]]
==Reference==
[[Category: Han K-H]]
NMR structure determination of alpha-conotoxin BuIA, a novel neuronal nicotinic acetylcholine receptor antagonist with an unusual 4/4 disulfide scaffold., Chi SW, Kim DH, Olivera BM, McIntosh JM, Han KH, Biochem Biophys Res Commun. 2006 Nov 3;349(4):1228-34. Epub 2006 Sep 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16979596 16979596]
[[Category: Kim D-H]]
[[Category: Single protein]]
[[Category: McIntosh JM]]
[[Category: Chi, S W.]]
[[Category: Olivera BM]]
[[Category: Han, K H.]]
[[Category: Kim, D H.]]
[[Category: McIntosh, J M.]]
[[Category: Olivera, B M.]]
[[Category: Alpha-helix]]
[[Category: Beta-turn]]
[[Category: C-terminal amidation]]
[[Category: Two disulfide bond]]
 
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Retrieved from "https://proteopedia.org/2i28"