Net charge: Difference between revisions
From Proteopedia
Jump to navigationJump to search
mNo edit summary |
Eric Martz (talk | contribs) |
||
| (3 intermediate revisions by 2 users not shown) | |||
| Line 2: | Line 2: | ||
<br>You can calculate the net charge of your protein easily at a given pH or over a range: | <br>You can calculate the net charge of your protein easily at a given pH or over a range: | ||
== | ==Protein Calculator== | ||
#First, get the one-letter amino acid sequence of your protein of interest. | #First, get the one-letter amino acid sequence of your protein of interest. | ||
##See the [[Isoelectric point|Isoelectric point page]] for some methods. | ##See the [[Isoelectric point|Isoelectric point page]] for some methods. | ||
##Or for a protein that has a | ##Or for a protein that has a 3D structure in Proteopedia, find the page for your structure named for PDB file code and click on [http://www.ebi.ac.uk/msd-srv/oca/oca-bin/ocamain OCA] under the structure. | ||
### At your PDB code in OCA, scroll down to Sequence-derived information (near the bottom). | ### At your PDB code in OCA, scroll down to Sequence-derived information (near the bottom). | ||
###Click on the link for the one-letter amino acid sequence for one chain. | ###Click on the link for the one-letter amino acid sequence for one chain. | ||
#Copy the sequence and paste it into the large box at Christopher Putnam's [http:// | #Copy the sequence and paste it into the large box at Christopher Putnam's [http://protcalc.sourceforge.net/ Protein Calculator]. | ||
#Check the first box under Charge at the right, and click Submit Query. | #Check the first box under Charge at the right, and click Submit Query. | ||
==Prot-pi== | |||
"Prot pi | Protein Tool calculates isoelectric point and net charge of proteins, as well as the exact mass and the absorption coefficient using the amino acid sequence." | |||
*Go to [https://www.protpi.ch/Calculator/ProteinTool Prot-pi]] and follow instructions. | |||
==See Also== | ==See Also== | ||
| Line 17: | Line 22: | ||
==Content Attribution== | ==Content Attribution== | ||
The calculation method was adapted from [http://www.umass.edu/molvis/workshop/osaka09m.htm a class syllabus taught by Eric Martz]. | The calculation method was adapted from [http://www.umass.edu/molvis/workshop/osaka09m.htm a class syllabus taught by Eric Martz]. | ||
[[Category:Topic Page]] | |||
