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New page: left|200px<br /><applet load="1diy" size="450" color="white" frame="true" align="right" spinBox="true" caption="1diy, resolution 3.0Å" /> '''CRYSTAL STRUCTURE OF ...
 
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[[Image:1diy.gif|left|200px]]<br /><applet load="1diy" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1diy, resolution 3.0&Aring;" />
'''CRYSTAL STRUCTURE OF ARACHIDONIC ACID BOUND IN THE CYCLOOXYGENASE ACTIVE SITE OF PGHS-1'''<br />


==Overview==
==CRYSTAL STRUCTURE OF ARACHIDONIC ACID BOUND IN THE CYCLOOXYGENASE ACTIVE SITE OF PGHS-1==
Prostaglandin H synthase-1 and -2 (PGHS-1 and -2) catalyze the committed, step in prostaglandin synthesis and are targets for nonsteroidal, anti-inflammatory drugs (NSAIDs) like aspirin. We have determined the, structure of PGHS-1 at 3 angstrom resolution with arachidonic acid (AA), bound in a chemically productive conformation. The fatty acid adopts an, extended L-shaped conformation that positions the 13proS hydrogen of AA, for abstraction by tyrosine-385, the likely radical donor. A space also, exists for oxygen addition on the antarafacial surface of the carbon in, the 11-position (C-11). While this conformation allows endoperoxide, formation between C-11 and C-9, it also implies that a subsequent, conformational rearrangement must occur to allow formation of the C-8/C-12, bond and to position C-15 for attack by a second molecule of oxygen.
<StructureSection load='1diy' size='340' side='right'caption='[[1diy]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1diy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ovis_aries Ovis aries]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DIY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DIY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACD:ARACHIDONIC+ACID'>ACD</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1diy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1diy OCA], [https://pdbe.org/1diy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1diy RCSB], [https://www.ebi.ac.uk/pdbsum/1diy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1diy ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PGH1_SHEEP PGH1_SHEEP] May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/di/1diy_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1diy ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Prostaglandin H synthase-1 and -2 (PGHS-1 and -2) catalyze the committed step in prostaglandin synthesis and are targets for nonsteroidal anti-inflammatory drugs (NSAIDs) like aspirin. We have determined the structure of PGHS-1 at 3 angstrom resolution with arachidonic acid (AA) bound in a chemically productive conformation. The fatty acid adopts an extended L-shaped conformation that positions the 13proS hydrogen of AA for abstraction by tyrosine-385, the likely radical donor. A space also exists for oxygen addition on the antarafacial surface of the carbon in the 11-position (C-11). While this conformation allows endoperoxide formation between C-11 and C-9, it also implies that a subsequent conformational rearrangement must occur to allow formation of the C-8/C-12 bond and to position C-15 for attack by a second molecule of oxygen.


==About this Structure==
The productive conformation of arachidonic acid bound to prostaglandin synthase.,Malkowski MG, Ginell SL, Smith WL, Garavito RM Science. 2000 Sep 15;289(5486):1933-7. PMID:10988074<ref>PMID:10988074</ref>
1DIY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Ovis_aries Ovis aries] with BOG, COH and ACD as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Prostaglandin-endoperoxide_synthase Prostaglandin-endoperoxide synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.99.1 1.14.99.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DIY OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
The productive conformation of arachidonic acid bound to prostaglandin synthase., Malkowski MG, Ginell SL, Smith WL, Garavito RM, Science. 2000 Sep 15;289(5486):1933-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10988074 10988074]
</div>
<div class="pdbe-citations 1diy" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Cyclooxygenase 3D structures|Cyclooxygenase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Ovis aries]]
[[Category: Ovis aries]]
[[Category: Prostaglandin-endoperoxide synthase]]
[[Category: Garavito RM]]
[[Category: Single protein]]
[[Category: Ginell SL]]
[[Category: Garavito, R.M.]]
[[Category: Malkowski MG]]
[[Category: Ginell, S.L.]]
[[Category: Smith WL]]
[[Category: Malkowski, M.G.]]
[[Category: Smith, W.L.]]
[[Category: ACD]]
[[Category: BOG]]
[[Category: COH]]
[[Category: arachidonic acid]]
[[Category: dioxygenase]]
[[Category: membrane protein]]
[[Category: peroxidase]]
 
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