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[[Image:1f99.gif|left|200px]]
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{{STRUCTURE_1f99|  PDB=1f99  |  SCENE=  }}
'''CRYSTAL STRUCTURE OF R-PHYCOCYANIN FROM POLYSIPHONIA AT 2.4 A RESOLUTION'''


==CRYSTAL STRUCTURE OF R-PHYCOCYANIN FROM POLYSIPHONIA AT 2.4 A RESOLUTION==
<StructureSection load='1f99' size='340' side='right'caption='[[1f99]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1f99]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Polysiphonia_urceolata Polysiphonia urceolata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F99 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F99 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BLA:BILIVERDINE+IX+ALPHA'>BLA</scene>, <scene name='pdbligand=CYC:PHYCOCYANOBILIN'>CYC</scene>, <scene name='pdbligand=PEB:PHYCOERYTHROBILIN'>PEB</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f99 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f99 OCA], [https://pdbe.org/1f99 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f99 RCSB], [https://www.ebi.ac.uk/pdbsum/1f99 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f99 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PHCA_POLUR PHCA_POLUR]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f9/1f99_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f99 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structure of R-phycocyanin from Polysiphonia urceolata (R-PC-PU) at 2.4 A is reported. The R-PC-PU crystal belongs to space group P4(3)2(1)2 with cell parameters a = 135.1 A, c = 210.0 A, and alpha = beta = gamma = 90 degrees. The structure was determined by molecular replacement. The crystallographic R-factor of the refined model is 0.189 (R(free) = 0.239). Comparison of the microenvironment of chromophore beta 155 in R-PC-PU and in C-PC from Fremyolla diphosiphon (C-PC-FD) reveals that their spectral differences may be caused by their different alpha 28 residues. In the R-PC-PU crystal structure, two (alpha beta)(3) trimers assemble face to face to form a hexamer, and two such hexamers assemble in two novel side-to-side arrangements. Possible models for the energy transfer from phycoerythrin to phycocyanin and from phycocyanin to allophycocyanin are proposed based on several phycobiliprotein crystal structures.


==Overview==
Crystal structure of R-phycocyanin and possible energy transfer pathways in the phycobilisome.,Jiang T, Zhang JP, Chang WR, Liang DC Biophys J. 2001 Aug;81(2):1171-9. PMID:11463658<ref>PMID:11463658</ref>
The crystal structure of R-phycocyanin from Polysiphonia urceolata (R-PC-PU) at 2.4 A is reported. The R-PC-PU crystal belongs to space group P4(3)2(1)2 with cell parameters a = 135.1 A, c = 210.0 A, and alpha = beta = gamma = 90 degrees. The structure was determined by molecular replacement. The crystallographic R-factor of the refined model is 0.189 (R(free) = 0.239). Comparison of the microenvironment of chromophore beta 155 in R-PC-PU and in C-PC from Fremyolla diphosiphon (C-PC-FD) reveals that their spectral differences may be caused by their different alpha 28 residues. In the R-PC-PU crystal structure, two (alpha beta)(3) trimers assemble face to face to form a hexamer, and two such hexamers assemble in two novel side-to-side arrangements. Possible models for the energy transfer from phycoerythrin to phycocyanin and from phycocyanin to allophycocyanin are proposed based on several phycobiliprotein crystal structures.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
1F99 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Polysiphonia_urceolata Polysiphonia urceolata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F99 OCA].
</div>
<div class="pdbe-citations 1f99" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Crystal structure of R-phycocyanin and possible energy transfer pathways in the phycobilisome., Jiang T, Zhang JP, Chang WR, Liang DC, Biophys J. 2001 Aug;81(2):1171-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11463658 11463658]
*[[Phycocyanin|Phycocyanin]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Polysiphonia urceolata]]
[[Category: Polysiphonia urceolata]]
[[Category: Protein complex]]
[[Category: Chang WR]]
[[Category: Chang, W R.]]
[[Category: Jiang T]]
[[Category: Jiang, T.]]
[[Category: Liang DC]]
[[Category: Liang, D C.]]
[[Category: Electron trasnport]]
[[Category: Light harvesting protein]]
[[Category: Photosynthesis]]
[[Category: R-phycocyanin]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 16:03:32 2008''

Latest revision as of 06:36, 30 October 2024

CRYSTAL STRUCTURE OF R-PHYCOCYANIN FROM POLYSIPHONIA AT 2.4 A RESOLUTION

1f99, resolution 2.40Å

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