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New page: left|200px<br /><applet load="1lb7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lb7" /> '''IGF-F1-1, A PEPTIDE ANTAGONIST OF IGF-1'''<b...
 
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[[Image:1lb7.jpg|left|200px]]<br /><applet load="1lb7" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1lb7" />
'''IGF-F1-1, A PEPTIDE ANTAGONIST OF IGF-1'''<br />


==Overview==
==IGF-F1-1, A PEPTIDE ANTAGONIST OF IGF-1==
A panel of 22 naive peptide libraries was constructed in a polyvalent, phage display format and sorted against insulin-like growth factor-1, (IGF-1). The libraries were pooled to achieve a total diversity of 4.4 x, 10(11). After three rounds of selection, the majority of the phage clones, bound specifically to IGF-1, with a disulfide-constrained CX(9)C scaffold, dominating the selection. Four monovalently displayed sub-libraries were, designed on the basis of these conserved motifs. Sub-library maturation in, a monovalent format yielded an antagonistic peptide that inhibited the, interactions between IGF-1 and two cell-surface receptors and those, between IGF-1 and two soluble IGF binding proteins with micromolar, potency. NMR analysis revealed that the peptide is highly structured in, the absence of IGF-1, and peptides that preorganize the binding elements, were selected during the sorting.
<StructureSection load='1lb7' size='340' side='right'caption='[[1lb7]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1lb7]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LB7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LB7 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lb7 OCA], [https://pdbe.org/1lb7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lb7 RCSB], [https://www.ebi.ac.uk/pdbsum/1lb7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lb7 ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A panel of 22 naive peptide libraries was constructed in a polyvalent phage display format and sorted against insulin-like growth factor-1 (IGF-1). The libraries were pooled to achieve a total diversity of 4.4 x 10(11). After three rounds of selection, the majority of the phage clones bound specifically to IGF-1, with a disulfide-constrained CX(9)C scaffold dominating the selection. Four monovalently displayed sub-libraries were designed on the basis of these conserved motifs. Sub-library maturation in a monovalent format yielded an antagonistic peptide that inhibited the interactions between IGF-1 and two cell-surface receptors and those between IGF-1 and two soluble IGF binding proteins with micromolar potency. NMR analysis revealed that the peptide is highly structured in the absence of IGF-1, and peptides that preorganize the binding elements were selected during the sorting.


==About this Structure==
Rapid identification of small binding motifs with high-throughput phage display: discovery of peptidic antagonists of IGF-1 function.,Deshayes K, Schaffer ML, Skelton NJ, Nakamura GR, Kadkhodayan S, Sidhu SS Chem Biol. 2002 Apr;9(4):495-505. PMID:11983338<ref>PMID:11983338</ref>
1LB7 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LB7 OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Rapid identification of small binding motifs with high-throughput phage display: discovery of peptidic antagonists of IGF-1 function., Deshayes K, Schaffer ML, Skelton NJ, Nakamura GR, Kadkhodayan S, Sidhu SS, Chem Biol. 2002 Apr;9(4):495-505. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11983338 11983338]
</div>
[[Category: Protein complex]]
<div class="pdbe-citations 1lb7" style="background-color:#fffaf0;"></div>
[[Category: Deshayes, K.]]
== References ==
[[Category: Kadkhodayan, S.]]
<references/>
[[Category: Nakamura, G.R.]]
__TOC__
[[Category: Schaffer, M.L.]]
</StructureSection>
[[Category: Sidhu, S.S.]]
[[Category: Large Structures]]
[[Category: Skelton, N.J.]]
[[Category: Deshayes K]]
[[Category: disulfide]]
[[Category: Kadkhodayan S]]
[[Category: loop-helix]]
[[Category: Nakamura GR]]
[[Category: peptide]]
[[Category: Schaffer ML]]
 
[[Category: Sidhu SS]]
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:45:36 2007''
[[Category: Skelton NJ]]
Retrieved from "https://proteopedia.org/1lb7"