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[[Image:2gfi.gif|left|200px]]


{{Structure
==Crystal structure of the phytase from D. castellii at 2.3 A==
|PDB= 2gfi |SIZE=350|CAPTION= <scene name='initialview01'>2gfi</scene>, resolution 2.290&Aring;
<StructureSection load='2gfi' size='340' side='right'caption='[[2gfi]], [[Resolution|resolution]] 2.29&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>
<table><tr><td colspan='2'>[[2gfi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Debaryomyces_castellii Debaryomyces castellii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GFI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GFI FirstGlance]. <br>
|ACTIVITY= [http://en.wikipedia.org/wiki/3-phytase 3-phytase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.8 3.1.3.8]  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.29&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gfi OCA], [https://pdbe.org/2gfi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gfi RCSB], [https://www.ebi.ac.uk/pdbsum/2gfi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gfi ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A2TBB4_9ASCO A2TBB4_9ASCO]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gf/2gfi_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gfi ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Phytate (myo-inositol hexakisphosphate) is the primary storage form of phosphate in seeds and legumes (Reddy et al., 1982). Phytases are phosphatases that hydrolyze phytate to less phosphorylated myo-inositol derivatives and inorganic phosphate. The crystal structure of phytase from Debaryomyces castellii has been determined at 2.3 A resolution. The crystals belonged to space group P6(5)22, with unit-cell parameters a = 121.65, c = 332.24 A. The structure was solved by molecular replacement and refined to a final R factor of 15.7% (R(free) = 20.9%). The final model consists of a dimer (with two monomers of 458 residues), five NAG molecules and 628 water molecules.


'''Crystal structure of the phytase from D. castellii at 2.3 A'''
Structure of Debaryomyces castellii CBS 2923 phytase.,Ragon M, Hoh F, Aumelas A, Chiche L, Moulin G, Boze H Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Apr 1;65(Pt, 4):321-6. Epub 2009 Mar 25. PMID:19342770<ref>PMID:19342770</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2gfi" style="background-color:#fffaf0;"></div>


==About this Structure==
==See Also==
2GFI is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Debaryomyces_castellii Debaryomyces castellii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GFI OCA].
*[[Phytase 3D structures|Phytase 3D structures]]
[[Category: 3-phytase]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Debaryomyces castellii]]
[[Category: Debaryomyces castellii]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: Hoh, F.]]
[[Category: Hoh F]]
[[Category: NAG]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:04:04 2008''
Retrieved from "https://proteopedia.org/2gfi"