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New page: left|200px<br /> <applet load="1f4y" size="450" color="white" frame="true" align="right" spinBox="true" caption="1f4y, resolution 2.8Å" /> '''CRYSTAL STRUCTURE OF...
 
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[[Image:1f4y.gif|left|200px]]<br />
<applet load="1f4y" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1f4y, resolution 2.8&Aring;" />
'''CRYSTAL STRUCTURE OF AN ANTI-CARBOHYDRATE ANTIBODY DIRECTED AGAINST VIBRIO CHOLERAE O1 IN COMPLEX WITH ANTIGEN'''<br />


==Overview==
==CRYSTAL STRUCTURE OF AN ANTI-CARBOHYDRATE ANTIBODY DIRECTED AGAINST VIBRIO CHOLERAE O1 IN COMPLEX WITH ANTIGEN==
The crystal structure of the murine Fab S-20-4 from a protective, anti-cholera Ab specific for the lipopolysaccharide Ag of the Ogawa, serotype has been determined in its unliganded form and in complex with, synthetic fragments of the Ogawa O-specific polysaccharide (O-SP). The, upstream terminal O-SP monosaccharide is shown to be the primary antigenic, determinant. Additional perosamine residues protrude outwards from the Ab, surface and contribute only marginally to the binding affinity and, specificity. A complementary water-excluding hydrophobic interface and, five Ab-Ag hydrogen bonds are crucial for carbohydrate recognition. The, structure reported here explains the serotype specificity of anti-Ogawa, Abs and provides a rational basis toward the development of a synthetic, carbohydrate-based anti-cholera vaccine.
<StructureSection load='1f4y' size='340' side='right'caption='[[1f4y]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1f4y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F4Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F4Y FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZCZ:4,6-dideoxy-4-{[(2R)-2,4-dihydroxybutanoyl]amino}-2-O-methyl-alpha-D-mannopyranose'>ZCZ</scene>, <scene name='pdbligand=ZD0:(2R)-N-[(2R,3S,4S,5S,6S)-6-methoxy-2-methyl-4,5-bis(oxidanyl)oxan-3-yl]-2,4-bis(oxidanyl)butanamide'>ZD0</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f4y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f4y OCA], [https://pdbe.org/1f4y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f4y RCSB], [https://www.ebi.ac.uk/pdbsum/1f4y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f4y ProSAT]</span></td></tr>
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f4/1f4y_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f4y ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structure of the murine Fab S-20-4 from a protective anti-cholera Ab specific for the lipopolysaccharide Ag of the Ogawa serotype has been determined in its unliganded form and in complex with synthetic fragments of the Ogawa O-specific polysaccharide (O-SP). The upstream terminal O-SP monosaccharide is shown to be the primary antigenic determinant. Additional perosamine residues protrude outwards from the Ab surface and contribute only marginally to the binding affinity and specificity. A complementary water-excluding hydrophobic interface and five Ab-Ag hydrogen bonds are crucial for carbohydrate recognition. The structure reported here explains the serotype specificity of anti-Ogawa Abs and provides a rational basis toward the development of a synthetic carbohydrate-based anti-cholera vaccine.


==About this Structure==
Crystal structure of an anti-carbohydrate antibody directed against Vibrio cholerae O1 in complex with antigen: molecular basis for serotype specificity.,Villeneuve S, Souchon H, Riottot MM, Mazie JC, Lei P, Glaudemans CP, Kovac P, Fournier JM, Alzari PM Proc Natl Acad Sci U S A. 2000 Jul 18;97(15):8433-8. PMID:10880560<ref>PMID:10880560</ref>
1F4Y is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with MGU as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1F4Y OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Crystal structure of an anti-carbohydrate antibody directed against Vibrio cholerae O1 in complex with antigen: molecular basis for serotype specificity., Villeneuve S, Souchon H, Riottot MM, Mazie JC, Lei P, Glaudemans CP, Kovac P, Fournier JM, Alzari PM, Proc Natl Acad Sci U S A. 2000 Jul 18;97(15):8433-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10880560 10880560]
</div>
<div class="pdbe-citations 1f4y" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Antibody 3D structures|Antibody 3D structures]]
*[[3D structures of non-human antibody|3D structures of non-human antibody]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Protein complex]]
[[Category: Alzari PM]]
[[Category: Alzari, P.M.]]
[[Category: Souchon H]]
[[Category: Souchon, H.]]
[[Category: MGU]]
[[Category: anti-carbohydrate antibody]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:29:47 2007''
Retrieved from "https://proteopedia.org/1f4y"