2bz1: Difference between revisions

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{{Seed}}
[[Image:2bz1.png|left|200px]]


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==CRYSTAL STRUCTURE OF APO E. COLI GTP CYCLOHYDROLASE II==
The line below this paragraph, containing "STRUCTURE_2bz1", creates the "Structure Box" on the page.
<StructureSection load='2bz1' size='340' side='right'caption='[[2bz1]], [[Resolution|resolution]] 1.54&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2bz1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BZ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BZ1 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.54&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TAU:2-AMINOETHANESULFONIC+ACID'>TAU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
{{STRUCTURE_2bz1|  PDB=2bz1  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bz1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bz1 OCA], [https://pdbe.org/2bz1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bz1 RCSB], [https://www.ebi.ac.uk/pdbsum/2bz1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bz1 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RIBA_ECOLI RIBA_ECOLI] Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.<ref>PMID:235552</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bz/2bz1_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bz1 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
GTP cyclohydrolase II converts GTP to 2,5-diamino-6-beta-ribosyl-4(3H)-pyrimidinone 5'-phosphate, formate and pyrophosphate, the first step in riboflavin biosynthesis. The essential role of riboflavin in metabolism and the absence of GTP cyclohydrolase II in higher eukaryotes makes it a potential novel selective antimicrobial drug target. GTP cyclohydrolase II catalyzes a distinctive overall reaction from GTP cyclohydrolase I; the latter converts GTP to dihydroneopterin triphosphate, utilized in folate and tetrahydrobiopterin biosynthesis. The structure of GTP cyclohydrolase II determined at 1.54-A resolution reveals both a different protein fold to GTP cyclohydrolase I and distinctive molecular recognition determinants for GTP; although in both enzymes there is a bound catalytic zinc. The GTP cyclohydrolase II.GMPCPP complex structure shows Arg(128) interacting with the alpha-phosphonate, and thus in the case of GTP, Arg(128) is positioned to act as the nucleophile for pyrophosphate release and formation of the proposed covalent guanylyl-GTP cyclohydrolase II intermediate. Tyr(105) is identified as playing a key role in GTP ring opening; it is hydrogen-bonded to the zinc-activated water molecule, the latter being positioned for nucleophilic attack on the guanine C-8 atom. Although GTP cyclohydrolase I and GTP cyclohydrolase II both use a zinc ion for the GTP ring opening and formate release, different residues are utilized in each case to catalyze this reaction step.


===CRYSTAL STRUCTURE OF APO E. COLI GTP CYCLOHYDROLASE II===
GTP cyclohydrolase II structure and mechanism.,Ren J, Kotaka M, Lockyer M, Lamb HK, Hawkins AR, Stammers DK J Biol Chem. 2005 Nov 4;280(44):36912-9. Epub 2005 Aug 22. PMID:16115872<ref>PMID:16115872</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2bz1" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_16115872}}, adds the Publication Abstract to the page
*[[Cyclohydrolase 3D structures|Cyclohydrolase 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 16115872 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_16115872}}
__TOC__
 
</StructureSection>
==About this Structure==
2BZ1 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BZ1 OCA].
 
==Reference==
<ref group="xtra">PMID:16115872</ref><references group="xtra"/>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: GTP cyclohydrolase II]]
[[Category: Large Structures]]
[[Category: Hawkins, A R.]]
[[Category: Hawkins AR]]
[[Category: Kotaka, M.]]
[[Category: Kotaka M]]
[[Category: Lamb, H K.]]
[[Category: Lamb HK]]
[[Category: Lockyer, M.]]
[[Category: Lockyer M]]
[[Category: Ren, J.]]
[[Category: Ren J]]
[[Category: Stammers, D K.]]
[[Category: Stammers DK]]
[[Category: Catalytic zinc]]
[[Category: Gtp cyclohydrolase ii]]
[[Category: Hydrolase]]
[[Category: Magnesium]]
[[Category: Riboflavin biosynthesis]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 14:47:09 2009''
Retrieved from "https://proteopedia.org/2bz1"