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[[Image:2g5b.gif|left|200px]]
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{{STRUCTURE_2g5b|  PDB=2g5b  |  SCENE=  }}
'''Crystal Structure of the anti-Bax monoclonal antibody 6A7 and a Bax peptide.'''


==Crystal Structure of the anti-Bax monoclonal antibody 6A7 and a Bax peptide.==
<StructureSection load='2g5b' size='340' side='right'caption='[[2g5b]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2g5b]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G5B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2G5B FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2g5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g5b OCA], [https://pdbe.org/2g5b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2g5b RCSB], [https://www.ebi.ac.uk/pdbsum/2g5b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2g5b ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HVM63_MOUSE HVM63_MOUSE]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g5/2g5b_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2g5b ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The Bcl-2 family member Bax plays a critical role in apoptosis. In healthy resting cells, Bax resides in the cytoplasm and loosely attached to the mitochondrial membrane. Apoptotic stimuli induce Bax activation, which is characterized by translocation and multimerization on the mitochondrial membrane surface resulting in exposure of an amino terminal epitope recognized by the monoclonal antibody 6A7. To understand the structural changes that occur during Bax activation, we determined the crystal structure of a Bax peptide bound to the 6A7 Fab fragment to a resolution of 2.3 A. The structure reveals the conformation of the 6A7 peptide epitope on Bax in the activated form and elucidates the extensive structural changes that Bax must undergo during the conversion from its native to its activated conformation.


==Overview==
Elucidation of some Bax conformational changes through crystallization of an antibody-peptide complex.,Peyerl FW, Dai S, Murphy GA, Crawford F, White J, Marrack P, Kappler JW Cell Death Differ. 2007 Mar;14(3):447-52. Epub 2006 Sep 1. PMID:16946732<ref>PMID:16946732</ref>
The Bcl-2 family member Bax plays a critical role in apoptosis. In healthy resting cells, Bax resides in the cytoplasm and loosely attached to the mitochondrial membrane. Apoptotic stimuli induce Bax activation, which is characterized by translocation and multimerization on the mitochondrial membrane surface resulting in exposure of an amino terminal epitope recognized by the monoclonal antibody 6A7. To understand the structural changes that occur during Bax activation, we determined the crystal structure of a Bax peptide bound to the 6A7 Fab fragment to a resolution of 2.3 A. The structure reveals the conformation of the 6A7 peptide epitope on Bax in the activated form and elucidates the extensive structural changes that Bax must undergo during the conversion from its native to its activated conformation.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
2G5B is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G5B OCA].
</div>
<div class="pdbe-citations 2g5b" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Elucidation of some Bax conformational changes through crystallization of an antibody-peptide complex., Peyerl FW, Dai S, Murphy GA, Crawford F, White J, Marrack P, Kappler JW, Cell Death Differ. 2007 Mar;14(3):447-52. Epub 2006 Sep 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16946732 16946732]
*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Protein complex]]
[[Category: Dai S]]
[[Category: Dai, S.]]
[[Category: Kappler JW]]
[[Category: Kappler, J W.]]
[[Category: Marrack P]]
[[Category: Marrack, P.]]
[[Category: Murphy GA]]
[[Category: Murphy, G A.]]
[[Category: Peyerl FW]]
[[Category: Peyerl, F W.]]
[[Category: 6a7]]
[[Category: Antibody]]
[[Category: Bax]]
[[Category: Fab]]
[[Category: Fab-peptide complex]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 04:42:08 2008''
Retrieved from "https://proteopedia.org/2g5b"