2qt4: Difference between revisions

From Proteopedia
Jump to navigationJump to search
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
New page: left|200px<br /><applet load="2qt4" size="350" color="white" frame="true" align="right" spinBox="true" caption="2qt4, resolution 1.300Å" /> '''Atomic-resolution c...
 
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
 
(14 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:2qt4.jpg|left|200px]]<br /><applet load="2qt4" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2qt4, resolution 1.300&Aring;" />
'''Atomic-resolution crystal structure of the natural form of Scytovirin'''<br />


==Overview==
==Atomic-resolution crystal structure of the natural form of Scytovirin==
The crystal structures of the natural and recombinant antiviral lectin, scytovirin (SVN) were solved by single-wavelength anomalous scattering and, refined with data extending to 1.3 A and 1.0 A resolution, respectively. A, molecule of SVN consists of a single chain 95 amino acids long, with an, almost perfect sequence repeat that creates two very similar domains (RMS, deviation 0.25 A for 40 pairs of Calpha atoms). The crystal structure, differs significantly from a previously published NMR structure of the, same protein, with the RMS deviations calculated separately for the N- and, C-terminal domains of 5.3 A and 3.7 A, respectively, and a very different, relationship between the two domains. In addition, the disulfide bonding, pattern of the crystal structures differs from that described in the, previously published mass spectrometry and NMR studies.
<StructureSection load='2qt4' size='340' side='right'caption='[[2qt4]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2qt4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Scytonema_varium Scytonema varium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QT4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QT4 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qt4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qt4 OCA], [https://pdbe.org/2qt4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qt4 RCSB], [https://www.ebi.ac.uk/pdbsum/2qt4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qt4 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SVN_SCYVA SVN_SCYVA] Has strong anti-HIV activity against T-tropic strains of HIV-1 and weaker activity against M-tropic strains of HIV-1. Inhibits HIV-1 fusion and infection of CD4 LTR beta-gal cells in vitro. Inhibits fusion of HIV infected CEM-SS cells with uninfected CEM-SS cells, and fusion of HIV-1 Env expressing HL2/3 cells with CD4 LTR beta-gal cells. Binds to HIV gp120, HIV gp160 and to a lesser extent HIV gp41. Binding to HIV gp120 is glycosylation dependent. Binds with high specificity to the tetrasaccharide Man-alpha-1,2-Man-alpha-1,6-Man-alpha-1,6-Man and also binds the higher-order oligosaccharides oligomannose 8 and oligomannose 9. Does not bind to monosaccharides, complex or hybrid N-linked oligosaccharides or chitin.<ref>PMID:12614152</ref> <ref>PMID:16647158</ref> <ref>PMID:17269926</ref> <ref>PMID:17434526</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structures of the natural and recombinant antiviral lectin scytovirin (SVN) were solved by single-wavelength anomalous scattering and refined with data extending to 1.3 A and 1.0 A resolution, respectively. A molecule of SVN consists of a single chain 95 amino acids long, with an almost perfect sequence repeat that creates two very similar domains (RMS deviation 0.25 A for 40 pairs of Calpha atoms). The crystal structure differs significantly from a previously published NMR structure of the same protein, with the RMS deviations calculated separately for the N- and C-terminal domains of 5.3 A and 3.7 A, respectively, and a very different relationship between the two domains. In addition, the disulfide bonding pattern of the crystal structures differs from that described in the previously published mass spectrometry and NMR studies.


==About this Structure==
Atomic-resolution crystal structure of the antiviral lectin scytovirin.,Moulaei T, Botos I, Ziolkowska NE, Bokesch HR, Krumpe LR, McKee TC, O'Keefe BR, Dauter Z, Wlodawer A Protein Sci. 2007 Dec;16(12):2756-60. Epub 2007 Oct 26. PMID:17965185<ref>PMID:17965185</ref>
2QT4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Scytonema_varium Scytonema varium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QT4 OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Atomic-resolution crystal structure of the antiviral lectin scytovirin., Moulaei T, Botos I, Ziolkowska NE, Bokesch HR, Krumpe LR, McKee TC, O'Keefe BR, Dauter Z, Wlodawer A, Protein Sci. 2007 Dec;16(12):2756-60. Epub 2007 Oct 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17965185 17965185]
</div>
[[Category: Protein complex]]
<div class="pdbe-citations 2qt4" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Scytonema varium]]
[[Category: Scytonema varium]]
[[Category: Botos, I.]]
[[Category: Botos I]]
[[Category: Dauter, Z.]]
[[Category: Dauter Z]]
[[Category: Moulaei, T.]]
[[Category: Moulaei T]]
[[Category: Wlodawer, A.]]
[[Category: Wlodawer A]]
[[Category: Ziolkowska, N.E.]]
[[Category: Ziolkowska NE]]
[[Category: lectin]]
[[Category: sugar binding protein]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:59:22 2008''
Retrieved from "https://proteopedia.org/2qt4"