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[[Image:2shk.jpg|left|200px]]
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{{STRUCTURE_2shk|  PDB=2shk  |  SCENE=  }}
'''THE THREE-DIMENSIONAL STRUCTURE OF SHIKIMATE KINASE FROM ERWINIA CHRYSANTHEMI COMPLEXED WITH ADP'''


==THE THREE-DIMENSIONAL STRUCTURE OF SHIKIMATE KINASE FROM ERWINIA CHRYSANTHEMI COMPLEXED WITH ADP==
<StructureSection load='2shk' size='340' side='right'caption='[[2shk]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2shk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dickeya_chrysanthemi Dickeya chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2SHK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2SHK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2shk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2shk OCA], [https://pdbe.org/2shk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2shk RCSB], [https://www.ebi.ac.uk/pdbsum/2shk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2shk ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/AROL_DICCH AROL_DICCH] Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate.<ref>PMID:11369852</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sh/2shk_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2shk ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Shikimate kinase from Erwinia chrysanthemi, overexpressed in Escherichia coli has been crystallized by the vapour-diffusion method using sodium chloride as a precipitant. Mass spectrometry was used to confirm the purity of the shikimate kinase and dynamic light scattering was used to assess conditions for the monodispersity of the enzyme. The crystals are tetragonal, space group P4(1)2(1)2 or enantiomorph with cell dimensions a = b = 108.5 and c = 92.8 A (at 100 K). Native crystals diffract to better than 2.6 A on a synchrotron X-ray source. The asymmetric unit is likely to contain two molecules, corresponding to a packing density of 3.6 A(3) Da(-1).


==Overview==
Crystallization and preliminary X-ray crystallographic analysis of shikimate kinase from Erwinia chrysanthemi.,Krell T, Coyle JE, Horsburgh MJ, Coggins JR, Lapthorn AJ Acta Crystallogr D Biol Crystallogr. 1997 Sep 1;53(Pt 5):612-4. PMID:15299895<ref>PMID:15299895</ref>
Shikimate kinase from Erwinia chrysanthemi, overexpressed in Escherichia coli has been crystallized by the vapour-diffusion method using sodium chloride as a precipitant. Mass spectrometry was used to confirm the purity of the shikimate kinase and dynamic light scattering was used to assess conditions for the monodispersity of the enzyme. The crystals are tetragonal, space group P4(1)2(1)2 or enantiomorph with cell dimensions a = b = 108.5 and c = 92.8 A (at 100 K). Native crystals diffract to better than 2.6 A on a synchrotron X-ray source. The asymmetric unit is likely to contain two molecules, corresponding to a packing density of 3.6 A(3) Da(-1).


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
2SHK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2SHK OCA].
</div>
<div class="pdbe-citations 2shk" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Crystallization and preliminary X-ray crystallographic analysis of shikimate kinase from Erwinia chrysanthemi., Krell T, Coyle JE, Horsburgh MJ, Coggins JR, Lapthorn AJ, Acta Crystallogr D Biol Crystallogr. 1997 Sep 1;53(Pt 5):612-4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15299895 15299895]
*[[Shikimate kinase 3D structures|Shikimate kinase 3D structures]]
[[Category: Erwinia chrysanthemi]]
== References ==
[[Category: Shikimate kinase]]
<references/>
[[Category: Single protein]]
__TOC__
[[Category: Coggins, J R.]]
</StructureSection>
[[Category: Krell, T.]]
[[Category: Dickeya chrysanthemi]]
[[Category: Lapthorn, A J.]]
[[Category: Large Structures]]
[[Category: Adp]]
[[Category: Coggins JR]]
[[Category: P-loop protein]]
[[Category: Krell T]]
[[Category: Phosphoryl transfer]]
[[Category: Lapthorn AJ]]
[[Category: Shikimate kinase]]
[[Category: Shikimate pathway]]
[[Category: Transferase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 17:18:57 2008''

Latest revision as of 09:30, 6 November 2024

THE THREE-DIMENSIONAL STRUCTURE OF SHIKIMATE KINASE FROM ERWINIA CHRYSANTHEMI COMPLEXED WITH ADP

2shk, resolution 2.60Å

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